Synthesis and molecular recognition of carbohydrate-centered multivalent glycoclusters by a plant lectin RCA120

被引：44

作者：

Gao, YJ

Eguchi, A

Kakehi, K

Lee, YC

机构：

[1] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

[2] Kinki Univ, Fac Pharmaceut Sci, Higashiosaka, Osaka 577, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2005年 / 13卷 / 22期

关键词：

lectin RCA(120); 1,3-cycloaddition reaction; glycocluster; molecular recognition;

D O I：

10.1016/j.bmc.2005.06.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Water soluble and lectin-recognizable carbohydrate-centered glycoclusters were prepared efficiently by the Huisgen 1,3-cycloaddition reaction of methyl-2,3,4,6-tetra-O-propargyl beta-D-galactopyranoside with 2-azidoethyl glycosides of lactose and N-acetyllactosamine. Their binding by a plant lectin RCA(120) was examined by capillary affinity electrophoresis using fluorescence-labeled asialoglycans from human alpha 1-acid glycoprotein. The glycoclusters showed 400-fold stronger inhibitory effect than free lactose, manifesting strong multivalency effect. (c) 2005 Elsevier Ltd. All rights reserved.

引用

收藏

页码：6151 / 6157

页数：7

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