A disposable amperometric biosensor for rapid screening of anticholinesterase activity in soil extracts

被引：51

作者：

Guerrieri, A

Monaci, L

Quinto, M

Palmisano, F

机构：

[1] Univ Bari, Dipartmento Chim, I-70126 Bari, Italy

[2] Univ Basilicata, Dipartimento Chim, I-85100 Potenza, Italy

[3] Univ Foggia, Ist Prod & Preparaz Alimentari, Fac Agr, I-71100 Foggia, Italy

来源：

ANALYST | 2002年 / 127卷 / 01期

关键词：

D O I：

10.1039/b109123a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A disposable amperometric biosensor for the determination of anticholinesterase activity in soil extracts is described. The sensitive membrane was obtained by co-crosslinking acetylcholinesterase and choline oxidase with bovine serum albumin using glutaraldehyde. The anticholinesterase activity of the soil extract was measured using chronoamperometry at 650 mV vs. Ag/AgCI to monitor the biocatalytically produced H2O2 before and after the inhibition step. An inhibition percentage of 38+/-4% was recorded for soil extracts spiked with 10 ppb of ethyl parathion. The device has the potential to be used as a gross sensor for the assessment of anticholinesterase activity in soil extracts.

引用

页码：5 / 7

页数：3

共 18 条

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