Nitric oxide ejects electrons from the binuclear centre of cytochrome c oxidase by reacting with oxidised copper: a general mechanism for the interaction of copper proteins with nitric oxide?

被引:100
作者
Cooper, CE
Torres, J
Sharpe, MA
Wilson, MT
机构
[1] Department of Biological Sciences, University of Essex, Central Campus, Colchester CO4 3SQ, Wivenhoe Park
基金
英国惠康基金;
关键词
nitric oxide; cytochrome oxidase; electron transfer; mitochondria; heme enzyme; copper enzyme;
D O I
10.1016/S0014-5793(97)01009-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Small increases in NO concentration can inhibit mitochondrial oxygen consumption by reacting at the binuclear haem a(3)/Cu-B oxygen reduction site of cytochrome c oxidase, Here we demonstrate that under normal turnover conditions NO reacts initially with the oxidised CUB rather than the haem a(3). We propose that hydration of an initial Cu+/NO+ complex forms nitrite, a proton and CUB+; the latter ejects an electron from the binuclear centre and results in the observed (100 s(-1)) reduction of other electron transfer centres in the enzyme (haem a and CUA) These reactions may have implications for the interactions of NO with other copper proteins. (C) 1997 Federation of European Biochemical Societies.
引用
收藏
页码:281 / 284
页数:4
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