Evanescent sensing of biomolecules and cells

被引：26

作者：

Haddock, HS

Shankar, PM

Mutharasan, R

机构：

[1] Drexel Univ, Dept Chem Engn, Philadelphia, PA 19104 USA

[2] Drexel Univ, Dept Elect & Comp Engn, Philadelphia, PA 19104 USA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2003年 / 88卷 / 01期

关键词：

NAD(P)H; microvolume; tapered fiber; cell concentration;

D O I：

10.1016/S0925-4005(02)00310-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A technique using the evanescent field of tapered fibers is developed for rapid, convenient, and accurate sensing of biomolecules and cells using small volumes of analytes in the range of 150 mul. A tapered optical fiber was fabricated by heat pulling with a flame. A simple fiber-mounting device was developed to accommodate the optical fiber and provide a reaction chamber for analytes to interact with the tapered region. Using an analytical grade spectrofluorometer nicotinamide adenine dinucleotide (NADH), nicotinamide adenine dinucleotide phosphate (NADPH), and Chinese Hamster Ovary (CHO) cells at various concentrations were measured. A parameter, namely, the product of extinction coefficient and light path, is used to characterize detection sensitivity. Results from biomolecules and cells show that the sensitivity of the tapered fiber is at least an order of magnitude higher than that obtained in a cuvette arrangement. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：67 / 74

页数：8

共 31 条

[11] NONINVASIVE SPECTROSCOPY FOR MONITORING CELL-DENSITY IN A FERMENTATION PROCESS [J].

GE, ZH ;

CAVINATO, AG ;

CALLIS, JB .

ANALYTICAL CHEMISTRY, 1994, 66 (08) :1354-1362

[12] FIBEROPTIC RETROREFLECTIVE TURBIDIMETER FOR CONTINUOUSLY MONITORING CELL CONCENTRATION DURING FERMENTATION [J].

HANCHER, CW ;

THACKER, LH ;

PHARES, EF .

BIOTECHNOLOGY AND BIOENGINEERING, 1974, 16 (04) :475-484

[13] Fiber optic evanescent field absorption sensor: Effect of fiber parameters and geometry of the probe [J].

Khijwania, SK ;

Gupta, BD .

OPTICAL AND QUANTUM ELECTRONICS, 1999, 31 (08) :625-636

[14] Chemically etched fiber tips for near-field optical microscopy: a process for smoother tips [J].

Lambelet, P ;

Sayah, A ;

Pfeffer, M ;

Philipona, C ;

Marquis-Weible, F .

APPLIED OPTICS, 1998, 37 (31) :7289-7292

[15] Optical fiber as a whole surface probe for chemical and biological applications [J].

Lin, WB ;

Jaffrezic-Renault, N ;

Chovelon, JM ;

Lacroix, M .

SENSORS AND ACTUATORS B-CHEMICAL, 2001, 74 (1-3) :207-211

[16] Bent silica fiber evanescent absorption sensors for near-infrared spectroscopy [J].

Littlejohn, D ;

Lucas, D ;

Han, L .

APPLIED SPECTROSCOPY, 1999, 53 (07) :845-849

[17] Optimizing the fabrication of aluminum-coated fiber probes and their application to optical near-field lithography [J].

Madsen, S ;

Holme, NCR ;

Ramanujam, PS ;

Hvilsted, S ;

Hvam, JM ;

Smith, SJ .

ULTRAMICROSCOPY, 1998, 71 (1-4) :65-71

[18] Development of a fibre optic micro-optrode for intracellular pH measurements [J].

McCulloch, S ;

Uttamchandani, D .

IEE PROCEEDINGS-OPTOELECTRONICS, 1997, 144 (03) :162-167

[19] Near-field optical apertured tip and modified structures for local field enhancement [J].

Minh, PN ;

Ono, T ;

Tanaka, S ;

Esashi, M .

APPLIED OPTICS, 2001, 40 (15) :2479-2484

[20] Reproducible fabrication of a fiber probe with a nanometric protrusion for near-field optics [J].

Mononobe, S ;

Naya, M ;

Saiki, T ;

Ohtsu, M .

APPLIED OPTICS, 1997, 36 (07) :1496-1500

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