Purification of gangliosides by liquid-liquid partition chromatography

被引：10

作者：

Kato, Tomohisa ^{[2
,3
]}

Hatanaka, Kenichi ^{[1
]}

机构：

[1] Univ Tokyo, Inst Ind Sci, Meguro Ku, Tokyo 1538505, Japan

[2] Japan Chem Innovat Inst, Chiyoda Ku, Tokyo 1010051, Japan

[3] Kaneka Corp, Kita Ku, Osaka 5308288, Japan

来源：

JOURNAL OF LIPID RESEARCH | 2008年 / 49卷 / 11期

关键词：

ganglioside; separation; centrifugal partition; chromatograph; counter-current chromatography;

D O I：

10.1194/jlr.D800033-JLR200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid-liquid partition chromatographic technique was applied to separate amphiphilic glycolipids. A two-phase solvent system composed of n-butanol-t-butyl methyl ether-acetonitrile-water at a volume ratio of 3:1:1:5 was found to be suitable for separating the gangliosides from total lipids extracted from rat brain by liquid-liquid partition chromatographic systems, namely centrifugal partition chromatography (CPC) and high-speed counter-current chromatography. GM1 could be separated rapidly by using the upper phase as stationary phase for both systems. Moreover, various kinds of gangliosides (GM1, GD1a, GD1b, GT1b) could be separated individually by using the lower phase as stationary phase by CPC. The sample can be recovered without loss by these systems.

引用

页码：2474 / 2478

页数：5

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