Phage display systems and their applications

被引:169
作者
Paschke, M [1 ]
机构
[1] Univ Med Berlin, Charite, Inst Biochem, D-10117 Berlin, Germany
关键词
D O I
10.1007/s00253-005-0270-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Screening phage display libraries of proteins and peptides has, for almost two decades, proven to be a powerful technology for selecting polypeptides with desired biological and physicochemical properties from huge molecular libraries. The scope of phage display applications continues to expand. Recent applications and technical improvements driving further developments in the field of phage display are discussed.
引用
收藏
页码:2 / 11
页数:10
相关论文
共 100 条
[1]   A 30-RESIDUE-LONG EXPORT INITIATION DOMAIN ADJACENT TO THE SIGNAL SEQUENCE IS CRITICAL FOR PROTEIN TRANSLOCATION ACROSS THE INNER MEMBRANE OF ESCHERICHIA-COLI [J].
ANDERSSON, H ;
VONHEIJNE, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) :9751-9754
[2]  
AYALA M, 1995, BIOTECHNIQUES, V18, P832
[3]  
Baca M, 1997, J BIOL CHEM, V272, P10678
[4]   An improved helper phage system for efficient isolation of specific antibody molecules in phage display [J].
Baek, H ;
Suk, KH ;
Kim, YH ;
Cha, S .
NUCLEIC ACIDS RESEARCH, 2002, 30 (05) :18
[5]   ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES - THE GENE-III SITE [J].
BARBAS, CF ;
KANG, AS ;
LERNER, RA ;
BENKOVIC, SJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (18) :7978-7982
[6]   The Tat protein export pathway [J].
Berks, BC ;
Sargent, F ;
Palmer, T .
MOLECULAR MICROBIOLOGY, 2000, 35 (02) :260-274
[7]   Small antibody-like proteins with prescribed ligand specificities derived from the lipocalin fold [J].
Beste, G ;
Schmidt, FS ;
Stibora, T ;
Skerra, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (05) :1898-1903
[8]   Engineered proteins as specific binding reagents [J].
Binz, HK ;
Plückthun, A .
CURRENT OPINION IN BIOTECHNOLOGY, 2005, 16 (04) :459-469
[9]   SINGLE-CHAIN ANTIGEN-BINDING PROTEINS [J].
BIRD, RE ;
HARDMAN, KD ;
JACOBSON, JW ;
JOHNSON, S ;
KAUFMAN, BM ;
LEE, SM ;
LEE, T ;
POPE, SH ;
RIORDAN, GS ;
WHITLOW, M .
SCIENCE, 1988, 242 (4877) :423-426
[10]   The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA -: I.: Increased functional expression of antibody fragments with and without cis-prolines [J].
Bothmann, H ;
Plückthun, A .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (22) :17100-17105