Phage display systems and their applications

被引:169
作者
Paschke, M [1 ]
机构
[1] Univ Med Berlin, Charite, Inst Biochem, D-10117 Berlin, Germany
关键词
D O I
10.1007/s00253-005-0270-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Screening phage display libraries of proteins and peptides has, for almost two decades, proven to be a powerful technology for selecting polypeptides with desired biological and physicochemical properties from huge molecular libraries. The scope of phage display applications continues to expand. Recent applications and technical improvements driving further developments in the field of phage display are discussed.
引用
收藏
页码:2 / 11
页数:10
相关论文
共 100 条
[51]   Selective cloning of peanut allergens, including profilin and 2S albumins, by phage display technology [J].
Kleber-Janke, T ;
Crameri, R ;
Appenzeller, U ;
Schlaak, M ;
Becker, WM .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1999, 119 (04) :265-274
[52]   Inclusion of an upstream transcriptional terminator in phage display vectors abolishes background expression of toxic fusions with coat protein g3p [J].
Krebber, A ;
Burmester, J ;
Pluckthun, A .
GENE, 1996, 178 (1-2) :71-74
[53]   Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system [J].
Krebber, A ;
Bornhauser, S ;
Burmester, J ;
Honegger, A ;
Willuda, J ;
Bosshard, HR ;
Pluckthun, A .
JOURNAL OF IMMUNOLOGICAL METHODS, 1997, 201 (01) :35-55
[54]  
KREBBER A, 2003, NUCLEIC ACIDS RES, V31, pE59
[55]   Co-selection of cognate antibody-antigen pairs by selectively-infective phages [J].
Krebber, C ;
Spada, S ;
Desplancq, D ;
Pluckthun, A .
FEBS LETTERS, 1995, 377 (02) :227-231
[56]   EVALUATION OF ANTIBODIES FUSED TO MINOR COAT PROTEIN-III AND MAJOR COAT PROTEIN-VIII OF BACTERIOPHAGE M13 [J].
KRETZSCHMAR, T ;
GEISER, M .
GENE, 1995, 155 (01) :61-65
[57]   Proteolytic selection for protein folding using filamentous bacteriophages [J].
Kristensen, P ;
Winter, G .
FOLDING & DESIGN, 1998, 3 (05) :321-328
[58]   In-vitro protein evolution by ribosome display and mRNA display [J].
Lipovsek, D ;
Plückthun, A .
JOURNAL OF IMMUNOLOGICAL METHODS, 2004, 290 (1-2) :51-67
[59]   BY-PASSING IMMUNIZATION - HUMAN-ANTIBODIES FROM V-GENE LIBRARIES DISPLAYED ON PHAGE [J].
MARKS, JD ;
HOOGENBOOM, HR ;
BONNERT, TP ;
MCCAFFERTY, J ;
GRIFFITHS, AD ;
WINTER, G .
JOURNAL OF MOLECULAR BIOLOGY, 1991, 222 (03) :581-597
[60]   Filamentous phage structure, infection and assembly [J].
Marvin, DA .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 1998, 8 (02) :150-158