EIN2-Directed Translational Regulation of Ethylene Signaling in Arabidopsis

被引：301

作者：

Li, Wenyang ^{[1
]}

Ma, Mengdi ^{[1
]}

Feng, Ying ^{[1
]}

Li, Hongjiang ^{[1
]}

Wang, Yichuan ^{[1
]}

Ma, Yutong ^{[1
]}

Li, Mingzhe ^{[1
]}

An, Fengying ^{[1
,2
]}

Guo, Hongwei ^{[1
,2
]}

机构：

[1] Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China

[2] Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China

来源：

CELL | 2015年 / 163卷 / 03期

基金：

中国国家自然科学基金;

关键词：

F-BOX PROTEINS; RESPONSE PATHWAY; ENDOPLASMIC-RETICULUM; TRANSDUCTION PATHWAY; CERNA HYPOTHESIS; RNA DECAY; EIN2; HORMONE; GAS; CTR1;

D O I：

10.1016/j.cell.2015.09.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ethylene is a gaseous phytohormone that plays vital roles in plant growth and development. Previous studies uncovered EIN2 as an essential signal transducer linking ethylene perception on ER to transcriptional regulation in the nucleus through a "cleave and shuttle'' model. In this study, we report another mechanism of EIN2-mediated ethylene signaling, whereby EIN2 imposes the translational repression of EBF1 and EBF2 mRNA. We find that the EBF1/2 3' UTRs mediate EIN2-directed translational repression and identify multiple poly-uridylates (PolyU) motifs as functional cis elements of 3' UTRs. Furthermore, we demonstrate that ethylene induces EIN2 to associate with 3' UTRs and target EBF1/2 mRNA to cytoplasmic processing-body (P-body) through interacting with multiple P-body factors, including EIN5 and PABs. Our study illustrates translational regulation as a key step in ethylene signaling and presents mRNA 3' UTR functioning as a "signal transducer'' to sense and relay cellular signaling in plants.

引用

页码：670 / 683

页数：14

共 42 条

[1] EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis [J].

Alonso, JM ;

Hirayama, T ;

Roman, G ;

Nourizadeh, S ;

Ecker, JR .

SCIENCE, 1999, 284 (5423) :2148-2152

[2] Ethylene-Induced Stabilization of ETHYLENE INSENSITIVE3 and EIN3-LIKE1 Is Mediated by Proteasomal Degradation of EIN3 Binding F-Box 1 and 2 That Requires EIN2 in Arabidopsis [J].

An, Fengying ;

Zhao, Qiong ;

Ji, Yusi ;

Li, Wenyang ;

Jiang, Zhiqiang ;

Yu, Xiangchun ;

Zhang, Chen ;

Han, Ying ;

He, Wenrong ;

Liu, Yidong ;

Zhang, Shuqun ;

Ecker, Joseph R. ;

Guo, Hongwei .

PLANT CELL, 2010, 22 (07) :2384-2401

[3] Targeting Plant Ethylene Responses by Controlling Essential Protein-Protein Interactions in the Ethylene Pathway [J].

Bisson, Melanie M. A. ;

Groth, Georg .

MOLECULAR PLANT, 2015, 8 (08) :1165-1174

[4]

Bisson MMA, 2011, PLANT SIGNAL BEHAV, V6, P164, DOI [10.4161/psb.6.1.14034, 10.4161/psb.5.12.14034]

[5] INSENSITIVITY TO ETHYLENE CONFERRED BY A DOMINANT MUTATION IN ARABIDOPSIS-THALIANA [J].

BLEECKER, AB ;

ESTELLE, MA ;

SOMERVILLE, C ;

KENDE, H .

SCIENCE, 1988, 241 (4869) :1086-1089

[6] Endogenous miRNA and Target Concentrations Determine Susceptibility to Potential ceRNA Competition [J].

Bosson, Andrew D. ;

Zamudio, Jesse R. ;

Sharp, Phillip A. .

MOLECULAR CELL, 2014, 56 (03) :347-359

[7] Molecular Titration and Ultrasensitivity in Regulatory Networks [J].

Buchler, Nicolas E. ;

Louis, Matthieu .

JOURNAL OF MOLECULAR BIOLOGY, 2008, 384 (05) :1106-1119

[8] Ethylene hormone receptor action in Arabidopsis [J].

Chang, C ;

Stadler, R .

BIOESSAYS, 2001, 23 (07) :619-627

[9] Temporal transcriptional response to ethylene gas drives growth hormone cross-regulation in Arabidopsis [J].

Chang, Katherine Noelani ;

Zhong, Shan ;

Weirauch, Matthew T. ;

Hon, Gary ;

Pelizzola, Mattia ;

Li, Hai ;

Huang, Shao-shan Carol ;

Schmitz, Robert J. ;

Urich, Mark A. ;

Kuo, Dwight ;

Nery, Joseph R. ;

Qiao, Hong ;

Yang, Ally ;

Jamali, Abdullah ;

Chen, Huaming ;

Ideker, Trey ;

Ren, Bing ;

Bar-Joseph, Ziv ;

Hughes, Timothy R. ;

Ecker, Joseph R. .

ELIFE, 2013, 2

[10] Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ETHYLENE-INSENSITIVE3 and related proteins [J].

Chao, QM ;

Rothenberg, M ;

Solano, R ;

Roman, G ;

Terzaghi, W ;

Ecker, JR .

CELL, 1997, 89 (07) :1133-1144

← 1 2 3 4 5 →