Label-free detection of cupric ions and histidine-tagged proteins using single poly(pyrrole)-NTA chelator conducting polymer nanotube chemiresistive sensor

被引：22

作者：

Aravinda, C. L. ^{[1
,2
]}

Cosnier, Serge ^{[3
]}

Chen, Wilfred ^{[1
,2
]}

Myung, Nosang V. ^{[1
,2
]}

Mulchandani, Ashok ^{[1
,2
]}

机构：

[1] Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA

[2] Univ Calif Riverside, Ctr Nanoscale Sci & Engn, Riverside, CA 92521 USA

[3] CNRS, Lab Electrochim Organ & Photochim Redox, UMR 5630, Inst Chim Mol Grenoble Fr 2607, F-38041 Grenoble 9, France

来源：

BIOSENSORS & BIOELECTRONICS | 2009年 / 24卷 / 05期

基金：

美国国家科学基金会;

关键词：

Nanotube; Histidine-tagged protein sensing; Poly(pyrrole)-NTA; Chemiresistive sensor; CHEMICAL DERIVATIZATION; FABRICATION;

D O I：

10.1016/j.bios.2008.08.044

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Novel chemical and biological sensors based on a single poly(pyrrole)-NTA chelator nanotube for sensitive, selective, rapid and real-time detection of histidine-tagged protein and cupric ions are reported. NTA groups on the nanotube surface provided a simple mechanism for metal ion sensing via the high-affinity interaction between NTA and the subsequent detection of histidine-tagged protein through the coordination with metal chelated nanotube. Poly(pyrrole)-NTA chelator nanotubes of 190 nm outside diameter, 35 nm wall thickness and 30 mu m long were synthesized by electrochemical polymerization of pyrrole-NTA inside a 200 nm diameter alumina template and assembled as a chemoresistive device by bottom-up contact geometry on a pair of parallel gold electrodes with a gap distance of 3 pm. The chemoresistive sensors based on single poly(pyrrole)-NTA chelator nanotube exhibited detection as low as one-hundredth attomolar (0.6 ppt) cupric ions and 1 ng/ml of penta-histidine tagged syntaxin protein. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：1451 / 1455

页数：5

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