TLR13 Recognizes Bacterial 23S rRNA Devoid of Erythromycin Resistance-Forming Modification

被引：310

作者：

Oldenburg, Marina ^{[1
]}

Krueger, Anne ^{[1
]}

Ferstl, Ruth ^{[2
]}

Kaufmann, Andreas ^{[3
]}

Nees, Gernot ^{[3
]}

Sigmund, Anna ^{[1
]}

Bathke, Barbara ^{[4
]}

Lauterbach, Henning ^{[4
]}

Suter, Mark ^{[4
,5
]}

Dreher, Stefan ^{[2
]}

Koedel, Uwe ^{[6
]}

Akira, Shizuo ^{[7
]}

Kawai, Taro ^{[7
]}

Buer, Jan ^{[1
]}

Wagner, Hermann ^{[2
]}

Bauer, Stefan ^{[3
]}

Hochrein, Hubertus

Kirschning, Carsten J. ^{[1
]}

机构：

[1] Univ Duisburg Essen, Inst Med Microbiol, D-45147 Essen, Germany

[2] Tech Univ Munich, Inst Med Microbiol Immunol & Hyg, D-81675 Munich, Germany

[3] Univ Marburg, Inst Immunol, D-35043 Marburg, Germany

[4] Bavarian Nord GmbH, Dept Res Immunol, D-82152 Martinsried, Germany

[5] Univ Zurich, Inst Virol, CH-8006 Zurich, Switzerland

[6] Clin Univ Munich, Dept Neurol, D-81377 Munich, Germany

[7] Osaka Univ, World Premier Int Immunol Frontier Res Ctr, Osaka 5650871, Japan

来源：

SCIENCE | 2012年 / 337卷 / 6098期

关键词：

TOLL-LIKE RECEPTORS; MACROLIDE RESISTANCE; DENDRITIC CELLS; LIPOPROTEINS; INFECTION; MUTATION; IMMUNITY; COMPLEX; SENSOR;

D O I：

10.1126/science.1220363

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Host protection from infection relies on the recognition of pathogens by innate pattern-recognition receptors such as Toll-like receptors (TLRs). Here, we show that the orphan receptor TLR13 in mice recognizes a conserved 23S ribosomal RNA (rRNA) sequence that is the binding site of macrolide, lincosamide, and streptogramin group (MLS) antibiotics (including erythromycin) in bacteria. Notably, 23S rRNA from clinical isolates of erythromycin-resistant Staphylococcus aureus and synthetic oligoribonucleotides carrying methylated adenosine or a guanosine mimicking a MLS resistance-causing modification failed to stimulate TLR13. Thus, our results reveal both a natural TLR13 ligand and specific mechanisms of antibiotic resistance as potent bacterial immune evasion strategy, avoiding recognition via TLR13.

引用

页码：1111 / 1115

页数：5

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