VEGF improves survival of mesenchymal stem cells in infarcted hearts

被引：127

作者：

Pons, Jennifer ^{[2
]}

Huang, Yu ^{[3
]}

Arakawa-Hoyt, Janice ^{[3
]}

Washko, Daniel ^{[3
]}

Takagawa, Junya ^{[4
]}

Ye, Jianqin ^{[4
]}

Grossman, William ^{[3
,4
]}

Su, Hua ^{[1
,2
]}

机构：

[1] Univ Calif San Francisco, Dept Anesthesia & Perioperat, San Francisco, CA 94110 USA

[2] Univ Calif San Francisco, Cardiovasc Res Inst, San Francisco, CA 94110 USA

[3] Univ Calif San Francisco, Dept Med, San Francisco, CA 94110 USA

[4] Univ Calif San Francisco, Div Cardiol, San Francisco, CA 94110 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 376卷 / 02期

基金：

美国国家卫生研究院;

关键词：

MSC; Cellular stress; VEGF; Myocardial infarction; Cell engraftment;

D O I：

10.1016/j.bbrc.2008.09.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bone marrow-derived mesenchymal stem cells (MSC) are a promising Source for cell-based treatment of myocardial infarction (MI), but existing strategies are restricted by low cell survival and engraftment. We examined whether vascular endothelial growth factor (VEGF) improve MSC viability in infracted hearts. We found long-term culture increased MSC-cellular stress: expressing more cell cycle inhibitors, p-16(INK), p21 and p19(ARF). VEGF treatment reduced cellular stress, increased pro-survival factors, phosphorylated-Akt and Bcl-xL expression and cell proliferation. Co-injection of MSCs with VEGF to MI hearts increased cell engraftment and resulted in better improvement of cardiac function than that injected with MSCs or VEGF alone. In conclusion, VEGF protects MSCs from culture-induce cellular stress and improves their viability in ischemic myocardium, which results in improvements of their therapeutic effect for the treatment of MI. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：419 / 422

页数：4

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