Investigation of biosensor signal bioamplification:: Comparison of direct electrochemistry phenomena of individual Laccase, and dual Laccase-Tyrosinase copper enzymes, at a Sonogel-Carbon electrode

被引：19

作者：

ElKaoutit, Mohammed ^{[1
]}

Naranjo-Rodriguez, Ignacio ^{[1
]}

Riffi Temsamani, Khalid ^{[2
]}

Dominguez, Manuel ^{[3
]}

Luis Hidalgo-Hidalgo de Cisneros, Jose ^{[1
]}

机构：

[1] Univ Cadiz, Fac Ciencias, Dept Quim Analit, Cadiz 11510, Spain

[2] Univ Abdelmalek Essaadi, Fac Siences Tetouan, Dept Chim, Equipe Rech Electrochim & Syst Interfaciaux, Mhannech 1193002, Morocco

[3] Univ Cadiz, Fac Ciencias, Dept Fis Mat Condensada, Cadiz 11510, Spain

来源：

TALANTA | 2008年 / 75卷 / 05期

关键词：

dual enzymes based biosensor; direct electrochemistry; signal bioamplification; Sonogel-Carbon electrode;

D O I：

10.1016/j.talanta.2008.01.055

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Direct electrochemistry of Trametes versicolor Laccase (LAC) was found at a Sonogel-Carbon electrode. The bioamplification, performed by dual immobilization of this enzyme and Mushroom Tyrosinase (TYR), of the bio-electrocatalytic reduction Of 02 was investigated. The calculated a transfer coefficients were 0.64 and 0.67, and the heterogeneous electron-transfer rate constants were 6.19 and 8.52 s(-1), respectively, for the individual LAC and dual LAC-TYR-based Nafion/Sonogel-Carbon bio-electrodes. The responses of the dual enzymes electrode to polyphenols were stronger than those of the individual LAC or TYR biosensors. Hypotheses are offered about the mechanism of bioamplification. The surfaces of the biosensors were also characterized by AFM. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：1348 / 1355

页数：8

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