Dnm3os, a Non-Coding RNA, Is Required for Normal Growth and Skeletal Development in Mice

被引：122

作者：

Watanabe, Tokumitsu ^{[1
,2
]}

Sato, Takahiro ^{[2
]}

Amano, Tomokazu ^{[2
]}

Kawamura, Yumiko ^{[2
]}

Kawamura, Naohiro ^{[3
]}

Kawaguchi, Hiroshi ^{[3
]}

Yamashita, Naohide ^{[1
]}

Kurihara, Hiroki ^{[2
]}

Nakaoka, Takashi ^{[1
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Dept Adv Med Sci, Tokyo 1088639, Japan

[2] Univ Tokyo, Grad Sch Med, Div Biochem & Mol Biol, Dept Physiol Chem & Metab, Tokyo, Japan

[3] Univ Tokyo, Fac Med, Dept Orthopaed Surg, Tokyo 1138654, Japan

来源：

DEVELOPMENTAL DYNAMICS | 2008年 / 237卷 / 12期

关键词：

non-coding RNA; micro RNA; skeletal development;

D O I：

10.1002/dvdy.21787

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Dnm3os, a gene that is transcribed into a non-coding RNA (ncRNA), contains three micro RNAs (miRNAs), miR-199a, miR-199a*, and miR-214, whose functions remain unknown in mammals. In this study, we introduced the lacZ gene into the Dnm3os locus to recapitulate its expression pattern and disrupt its function. Dnm3os(+/lacZ) heterozygous embryos showed beta-galactosidase activity, which reflected the authentic expression pattern of Dnm3os RNA. Most of the Dnm3os(lacZ/lacZ) homozygous pups died within one month of birth. After birth, Dnm3os(lacZ/lacZ) mice exhibited several skeletal abnormalities, including craniofacial hypoplasia, defects in dorsal neural arches and spinous processes of the vertebrae, and osteopenia. Importantly, the expression of miR-199a, miR-199a*, and miR-214 was significantly down-regulated in Dnm3os(lacZ/lacZ) embryos, supporting the assumption that Dnm3os serves as a precursor of these three miRNAs. Thus, Dnm3os has emerged as an miRNA-encoding gene that is indispensable for normal skeletal development and body growth in mammals. Developmental Dynamics 237:3738-3748, 2008. (c) 2008 Wiley-Liss, Inc.

引用

页码：3738 / 3748

页数：11

共 35 条

[1] Probing microRNAs with microarrays: Tissue specificity and functional inference [J].

Babak, T ;

Zhang, W ;

Morris, Q ;

Blencowe, BJ ;

Hughes, TR .

RNA, 2004, 10 (11) :1813-1819

[2] Differential distribution of dynamin isoforms in mammalian cells [J].

Cao, H ;

Garcia, F ;

McNiven, MA .

MOLECULAR BIOLOGY OF THE CELL, 1998, 9 (09) :2595-2609

[3] The transcriptional landscape of the mammalian genome [J].

Carninci, P ;

Kasukawa, T ;

Katayama, S ;

Gough, J ;

Frith, MC ;

Maeda, N ;

Oyama, R ;

Ravasi, T ;

Lenhard, B ;

Wells, C ;

Kodzius, R ;

Shimokawa, K ;

Bajic, VB ;

Brenner, SE ;

Batalov, S ;

Forrest, ARR ;

Zavolan, M ;

Davis, MJ ;

Wilming, LG ;

Aidinis, V ;

Allen, JE ;

Ambesi-Impiombato, X ;

Apweiler, R ;

Aturaliya, RN ;

Bailey, TL ;

Bansal, M ;

Baxter, L ;

Beisel, KW ;

Bersano, T ;

Bono, H ;

Chalk, AM ;

Chiu, KP ;

Choudhary, V ;

Christoffels, A ;

Clutterbuck, DR ;

Crowe, ML ;

Dalla, E ;

Dalrymple, BP ;

de Bono, B ;

Della Gatta, G ;

di Bernardo, D ;

Down, T ;

Engstrom, P ;

Fagiolini, M ;

Faulkner, G ;

Fletcher, CF ;

Fukushima, T ;

Furuno, M ;

Futaki, S ;

Gariboldi, M .

SCIENCE, 2005, 309 (5740) :1559-1563

[4] Antisense inhibition of human miRNAs and indications for an involvement of miRNA in cell growth and apoptosis [J].

Cheng, AM ;

Byrom, MW ;

Shelton, J ;

Ford, LP .

NUCLEIC ACIDS RESEARCH, 2005, 33 (04) :1290-1297

[5] Transcriptional maps of 10 human chromosomes at 5-nucleotide resolution [J].

Cheng, J ;

Kapranov, P ;

Drenkow, J ;

Dike, S ;

Brubaker, S ;

Patel, S ;

Long, J ;

Stern, D ;

Tammana, H ;

Helt, G ;

Sementchenko, V ;

Piccolboni, A ;

Bekiranov, S ;

Bailey, DK ;

Ganesh, M ;

Ghosh, S ;

Bell, I ;

Gerhard, DS ;

Gingeras, TR .

SCIENCE, 2005, 308 (5725) :1149-1154

[6] MicroRNAs are aberrantly expressed in hypertrophic heart - Do they play a role in cardiac hypertrophy? [J].

Cheng, Yunhui ;

Ji, Ruirui ;

Yue, Junming ;

Yang, Jian ;

Liu, Xiaojun ;

Chen, He ;

Dean, David B. ;

Zhang, Chunxiang .

AMERICAN JOURNAL OF PATHOLOGY, 2007, 170 (06) :1831-1840

[7] Comparative sequence analysis of the X-inactivation center region in mouse, human, and bovine [J].

Chureau, C ;

Prissette, M ;

Bourdet, A ;

Barbe, V ;

Cattolico, L ;

Jones, L ;

Eggen, A ;

Avner, P ;

Duret, L .

GENOME RESEARCH, 2002, 12 (06) :894-908

[8] Zebrafish miR-214 modulates Hedgehog signaling to specify muscle cell fate [J].

Flynt, Alex S. ;

Li, Nan ;

Thatcher, Elizabeth J. ;

Solnica-Krezel, Lilianna ;

Patton, James G. .

NATURE GENETICS, 2007, 39 (02) :259-263

[9] A diastrophic dysplasia sulfate transporter (SLC26A2) mutant mouse:: morphological and biochemical characterization of the resulting chondrodysplasia phenotype [J].

Forlino, A ;

Piazza, R ;

Torre, SD ;

Tatangelo, L ;

Bonafè, L ;

Gualeni, B ;

Romano, A ;

Pecora, F ;

Superti-Furga, A ;

Cetta, G ;

Rossi, A .

HUMAN MOLECULAR GENETICS, 2005, 14 (06) :859-871

[10] RETRACTED: DEAD-box RNA helicase subunits of the Drosha complex are required for processing of rRNA and a subset of microRNAs (Retracted article. See vol. 16, pg. 1126, 2014) [J].

Fukuda, Toru ;

Yamagata, Kaoru ;

Fujiyama, Sally ;

Matsumoto, Takahiro ;

Koshida, Iori ;

Yoshimura, Kimihiro ;

Mihara, Masatomo ;

Naitou, Masanori ;

Endoh, Hideki ;

Nakamura, Takashi ;

Akimoto, Chihiro ;

Yamamoto, Yoko ;

Katagiri, Takenobu ;

Foulds, Charles ;

Takezawa, Shinichiro ;

Kitagawa, Hirochika ;

Takeyama, Ken-ichi ;

O'Malley, Bert W. ;

Kato, Shigeaki .

NATURE CELL BIOLOGY, 2007, 9 (05) :604-U221

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