Dnm3os, a Non-Coding RNA, Is Required for Normal Growth and Skeletal Development in Mice

被引：122

作者：

Watanabe, Tokumitsu ^{[1
,2
]}

Sato, Takahiro ^{[2
]}

Amano, Tomokazu ^{[2
]}

Kawamura, Yumiko ^{[2
]}

Kawamura, Naohiro ^{[3
]}

Kawaguchi, Hiroshi ^{[3
]}

Yamashita, Naohide ^{[1
]}

Kurihara, Hiroki ^{[2
]}

Nakaoka, Takashi ^{[1
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Dept Adv Med Sci, Tokyo 1088639, Japan

[2] Univ Tokyo, Grad Sch Med, Div Biochem & Mol Biol, Dept Physiol Chem & Metab, Tokyo, Japan

[3] Univ Tokyo, Fac Med, Dept Orthopaed Surg, Tokyo 1138654, Japan

来源：

DEVELOPMENTAL DYNAMICS | 2008年 / 237卷 / 12期

关键词：

non-coding RNA; micro RNA; skeletal development;

D O I：

10.1002/dvdy.21787

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Dnm3os, a gene that is transcribed into a non-coding RNA (ncRNA), contains three micro RNAs (miRNAs), miR-199a, miR-199a*, and miR-214, whose functions remain unknown in mammals. In this study, we introduced the lacZ gene into the Dnm3os locus to recapitulate its expression pattern and disrupt its function. Dnm3os(+/lacZ) heterozygous embryos showed beta-galactosidase activity, which reflected the authentic expression pattern of Dnm3os RNA. Most of the Dnm3os(lacZ/lacZ) homozygous pups died within one month of birth. After birth, Dnm3os(lacZ/lacZ) mice exhibited several skeletal abnormalities, including craniofacial hypoplasia, defects in dorsal neural arches and spinous processes of the vertebrae, and osteopenia. Importantly, the expression of miR-199a, miR-199a*, and miR-214 was significantly down-regulated in Dnm3os(lacZ/lacZ) embryos, supporting the assumption that Dnm3os serves as a precursor of these three miRNAs. Thus, Dnm3os has emerged as an miRNA-encoding gene that is indispensable for normal skeletal development and body growth in mammals. Developmental Dynamics 237:3738-3748, 2008. (c) 2008 Wiley-Liss, Inc.

引用

页码：3738 / 3748

页数：11

共 35 条

[21] Know your neighbors: Three phenotypes in null mutants of the myogenic bHLH gene MRF4 [J].

Olson, EN ;

Arnold, HH ;

Rigby, PWJ ;

Wold, BJ .

CELL, 1996, 85 (01) :1-4

[22] The mammalian twisted gastrulation gene functions in foregut and craniofacial development [J].

Petryk, A ;

Anderson, RM ;

Jarcho, MP ;

Leaf, H ;

Carlson, CS ;

Klingensmith, J ;

Shawlot, W ;

O'Connor, MB .

DEVELOPMENTAL BIOLOGY, 2004, 267 (02) :374-386

[23] The dynamin superfamily: Universal membrane tubulation and fission molecules? [J].

Praefcke, GJK ;

McMahon, HT .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2004, 5 (02) :133-147

[24] Illuminating the silence: understanding the structure and function of small RNAs [J].

Rana, Tariq M. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2007, 8 (01) :23-36

[25] A mouse atlas of gene expression: Large-scale digital gene-expression profiles from precisely defined developing C57BL/6J mouse tissues and cells [J].

Siddiqui, AS ;

Khattra, J ;

Delaney, AD ;

Zhao, YJ ;

Astell, C ;

Asano, J ;

Babakaiff, R ;

Barber, S ;

Beland, J ;

Bohacec, S ;

Brown-John, M ;

Chand, S ;

Charest, D ;

Charters, AM ;

Cullum, R ;

Dhalla, N ;

Featherstone, R ;

Gerhard, DS ;

Hoffman, B ;

Holt, RA ;

Hou, J ;

Kuo, BYL ;

Lee, LLC ;

Lee, S ;

Leung, D ;

Ma, K ;

Matsuo, C ;

Mayo, M ;

McDonald, H ;

Prabhu, AI ;

Pandoh, P ;

Riggins, GJ ;

de Algara, TR ;

Rupert, JL ;

Smailus, D ;

Stott, J ;

Tsai, M ;

Varhol, R ;

Vrljicak, P ;

Wong, D ;

Wu, MK ;

Xie, YY ;

Yang, G ;

Zhang, I ;

Hirst, M ;

Jones, SJM ;

Helgason, CD ;

Simpson, EM ;

Hoodless, PA ;

Marra, MA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (51) :18485-18490

[26] The non-coding Air RNA is required for silencing autosomal imprinted genes [J].

Sleutels, F ;

Zwart, R ;

Barlow, DP .

NATURE, 2002, 415 (6873) :810-813

[27] The role of dynamin 3 in the testis [J].

Vaid, K. S. ;

Guttman, J. A. ;

Babyak, N. ;

Deng, W. ;

McNiven, M. A. ;

Mochizuki, N. ;

Finlay, B. B. ;

Vogl, A. W. .

JOURNAL OF CELLULAR PHYSIOLOGY, 2007, 210 (03) :644-654

[28] A signature pattern of stress-responsive microRNAs that can evoke cardiac hypertrophy and heart failure [J].

van Rooij, Eva ;

Sutherland, Lillian B. ;

Liu, Ning ;

Williams, Andrew H. ;

McAnally, John ;

Gerard, Robert D. ;

Richardson, James A. ;

Olson, Eric N. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (48) :18255-18260

[29] MicroRNA expression in zebrafish embryonic development [J].

Wienholds, E ;

Kloosterman, WP ;

Miska, E ;

Alvarez-Saavedra, E ;

Berezikov, E ;

de Bruijn, E ;

Horvitz, HR ;

Kauppinen, S ;

Plasterk, RHA .

SCIENCE, 2005, 309 (5732) :310-311

[30] A strategy for probing the function of noncoding RNAs finds a repressor of NFAT [J].

Willingham, AT ;

Orth, AP ;

Batalov, S ;

Peters, EC ;

Wen, BG ;

Aza-Blanc, P ;

Hogenesch, JB ;

Schultz, PG .

SCIENCE, 2005, 309 (5740) :1570-1573

← 1 2 3 4 →