Immobilization of whole cell penicillin G acylase in open pore gelatin matrix

被引：33

作者：

Norouzian, D ^{[1
]}

Javadpour, S

Moazami, N

Akbarzadeh, A

机构：

[1] Pasteur Inst Iran, Pilot Biotechnol Dept, Tehran 13164, IR, Iran

[2] IROST, Biotechnol Res Ctr, Tehran, IR, Iran

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2002年 / 30卷 / 01期

关键词：

whole cell penicillin G acylase; immobilization;

D O I：

10.1016/S0141-0229(01)00445-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Permeabilization of the cells of E. coli with N-cetyl-N, N, N-trimethylammoniumbromide (CTAB) showed 40% increase in penicillin G acylase activity. The treated cells were immobilized in porous beads of gelatin cross-linked with glutaraldehyde. Upon entrapment the catalytic properties of enzyme were changed as compared to non-immobilized form. Hydrolysis of penicillin G by immobilized system showed 60% conversion of substrate to 6-aminopenicillanic acid in batch system, whereas in continuous system, the conversion rate was 85%. The immobilized form of whole cell penicillin acylase showed low K-m toward penicillin G as a substrate. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：26 / 29

页数：4

共 10 条

[1] ISOLATION AND KINETICS OF PENICILLIN AMIDASE FROM ESCHERICHIA-COLI [J].

BALASINGHAM, K ;

WARBURTON, D ;

DUNNILL, P ;

LILLY, MD .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 276 (01) :250-+

[2] PURIFICATION AND PROPERTIES OF PENICILLIN AMIDASE FROM BACILLUS MEGATERIUM [J].

CHIANG, C ;

BENNETT, RE .

JOURNAL OF BACTERIOLOGY, 1967, 93 (01) :302-&

[3]

ERARSLAN A, 1991, J CHEM TECHNOL BIOT, V51, P27

[4]

ERARSLAN A, 1992, J CHEM TECHNOL BIOT, V55, P79

[5]

ERARSLAN A, 1990, DOGA T J ENG ENV SCI, V14, P381

[6]

LIANGTZUNG H, 1997, J APPL BIOCH BIOTECH, V62, P303

[7] Various techniques used to immobilize naringinase produced by Penicillium decombens PTCC 5248 [J].

Norouzian, D ;

Hosseinzadeh, A ;

Inanlou, DN ;

Moazami, N .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1999, 15 (04) :501-502

[8] IMMOBILIZATION OF PERMEABILIZED ESCHERICHIA-COLI-CELLS WITH PENICILLIN ACYLASE ACTIVITY [J].

PRABHUNE, AA ;

RAO, BS ;

PUNDLE, AV ;

SIVARAMAN, H .

ENZYME AND MICROBIAL TECHNOLOGY, 1992, 14 (02) :161-163

[9] CONTINUOUS ETHANOL-PRODUCTION BY YEAST-CELLS IMMOBILIZED IN OPEN PORE GELATIN MATRIX [J].

SIVARAMAN, H ;

RAO, BS ;

PUNDLE, AV ;

SIVARAMAN, C .

BIOTECHNOLOGY LETTERS, 1982, 4 (06) :359-364

[10] MOLECULAR ASPECTS OF PENICILLIN AND CEPHALOSPORIN ACYLASES [J].

SUDHAKARAN, VK ;

DESHPANDE, BS ;

AMBEDKAR, SS ;

SHEWALE, JG .

PROCESS BIOCHEMISTRY, 1992, 27 (03) :131-143

← 1 →