Development of a rapid and simple Agrobacterium tumefaciens-mediated transformation system for the fungal pathogen Heterobasidion annosum

被引：12

作者：

Samils, N

Elfstrand, M

Czederpiltz, DLL

Fahleson, J

Olson, Å

Dixelius, C

Stenlid, J

机构：

[1] Swedish Univ Agr Sci, Dept Forest Mycol & Pathol, S-75007 Uppsala, Sweden

[2] USDA, Forest Prod Lab, Ctr Forest Mycol Res, Madison, WI USA

[3] Swedish Univ Agr Sci, Dept Plant Biol & Forest Genet, Uppsala, Sweden

来源：

FEMS MICROBIOLOGY LETTERS | 2006年 / 255卷 / 01期

关键词：

Agrobacterium tumefaciens; Heterobasidion annosum; green fluorescent protein (GFP); transformation;

D O I：

10.1111/j.1574-6968.2005.00069.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Heterobasidion annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion annosum TC 32-1 was cocultivated at pH 5.6 and 20 degrees C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity.

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页码：82 / 88

页数：7

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