SirT1 mediates hyperbaric oxygen preconditioning-induced ischemic tolerance in rat brain

被引：151

作者：

Yan, Wenjun ^{[1
,2
]}

Fang, Zongping ^{[1
]}

Yang, Qianzi ^{[1
]}

Dong, Hailong ^{[1
]}

Lu, Yan ^{[1
]}

Lei, Chong ^{[1
]}

Xiong, Lize ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Anesthesiol, Xian 710032, Shaanxi Provinc, Peoples R China

[2] Gansu Prov Hosp, Dept Anesthesiol, Lanzhou, Peoples R China

来源：

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM | 2013年 / 33卷 / 03期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

cerebral ischemia/reperfusion; hyperbaric oxygen preconditioning; neuroprotection; oxygen-glucose deprivation; SirT1; CEREBRAL-ARTERY OCCLUSION; SPINAL-CORD ISCHEMIA; OXIDATIVE STRESS; UP-REGULATION; CALORIE RESTRICTION; HISTONE DEACETYLASE; ANTIOXIDANT ENZYMES; CELL-SURVIVAL; LIFE-SPAN; RESVERATROL;

D O I：

10.1038/jcbfm.2012.179

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Our previous studies have shown that hyperbaric oxygen preconditioning (HBO-PC) induces tolerance to cerebral ischemia/reperfusion (I/R). This study aimed to investigate whether SirT1, a class III histone deacetylase, is involved in neuroprotection elicited by HBO-PC in animal and cell culture models of ischemia. Rats were subjected to middle cerebral artery occlusion for 120 minutes after HBO-PC (once a day for 5 days). Primary cultured cortical neurons were exposed to 2 hours of HBO-PC after 2 hours of oxygen-glucose deprivation (OGD). We showed that HBO-PC increased SirT1 protein and mRNA expression, promoted neurobehavioral score, reduced infarct volume, and improved morphology at 24 hours and 7 days after cerebral I/R. Neuroprotection of HBO-PC was attenuated by SirT1 inhibitor EX527 and SirT1 knockdown by short interfering RNA (siRNA), whereas it was mimicked by SirT1 activator resveratrol. Furthermore, HBO-PC enhanced SirT1 expression and cell viability and reduced lactate dehydrogenase release 24 hours after OGD/re-oxygenation. The neuroprotective effect of HBO-PC was emulated through upregulating SirT1 and, reversely, attenuated through downregulating SirT1. The modulation of SirT1 was made by adenovirus infection carrying SirT1 or SirT1 siRNA. Besides, SirT1 increased B-cell lymphoma 2 (Bcl-2) expression and decrease cleaved caspase 3. These results indicate that SirT1 mediates HBO-PC-induced tolerance to cerebral I/R through inhibition of apoptosis. Journal of Cerebral Blood Flow & Metabolism (2013) 33, 396-406; doi: 10.1038/jcbfm.2012.179; published online 9 January 2013

引用

页码：396 / 406

页数：11

共 41 条

[1]

Silent information regulator 2α, a longevity factor and class III histone deacetylase, is an essential endogenous apoptosis inhibitor in cardiac myocytes [J].

Alcendor, RR ;

Kirshenbaum, LA ;

Imai, S ;

Vatner, SF ;

Sadoshima, J .

CIRCULATION RESEARCH, 2004, 95 (10) :971-980

[2]

Core and penumbral nitric oxide synthase activity during cerebral ischemia and reperfusion [J].

Ashwal, S ;

Tone, B ;

Tian, HR ;

Cole, DJ ;

Pearce, WJ .

STROKE, 1998, 29 (05) :1037-1046

[3]

Mitochondria, oxidants, and aging [J].

Balaban, RS ;

Nemoto, S ;

Finkel, T .

CELL, 2005, 120 (04) :483-495

[4]

Resveratrol improves health and survival of mice on a high-calorie diet [J].

Baur, Joseph A. ;

Pearson, Kevin J. ;

Price, Nathan L. ;

Jamieson, Hamish A. ;

Lerin, Carles ;

Kalra, Avash ;

Prabhu, Vinayakumar V. ;

Allard, Joanne S. ;

Lopez-Lluch, Guillermo ;

Lewis, Kaitlyn ;

Pistell, Paul J. ;

Poosala, Suresh ;

Becker, Kevin G. ;

Boss, Olivier ;

Gwinn, Dana ;

Wang, Mingyi ;

Ramaswamy, Sharan ;

Fishbein, Kenneth W. ;

Spencer, Richard G. ;

Lakatta, Edward G. ;

Le Couteur, David ;

Shaw, Reuben J. ;

Navas, Placido ;

Puigserver, Pere ;

Ingram, Donald K. ;

de Cabo, Rafael ;

Sinclair, David A. .

NATURE, 2006, 444 (7117) :337-342

[5]

The Sir2 family of protein deacetylases [J].

Blander, G ;

Guarente, L .

ANNUAL REVIEW OF BIOCHEMISTRY, 2004, 73 :417-435

[6]

SIRT1 transgenic mice show phenotypes resembling calorie restriction [J].

Bordone, Laura ;

Cohen, Dena ;

Robinson, Ashley ;

Motta, Maria Carla ;

van Veen, Ed ;

Czopik, Agnieszka ;

Steele, Andrew D. ;

Crowe, Hayley ;

Marmor, Stephen ;

Luo, Jianyuan ;

Gu, Wei ;

Guarente, Leonard .

AGING CELL, 2007, 6 (06) :759-767

[7]

Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase [J].

Brunet, A ;

Sweeney, LB ;

Sturgill, JF ;

Chua, KF ;

Greer, PL ;

Lin, YX ;

Tran, H ;

Ross, SE ;

Mostoslavsky, R ;

Cohen, HY ;

Hu, LS ;

Cheng, HL ;

Jedrychowski, MP ;

Gygi, SP ;

Sinclair, DA ;

Alt, FW ;

Greenberg, ME .

SCIENCE, 2004, 303 (5666) :2011-2015

[8]

Resveratrol protects cardiomyocytes from hypoxia-induced apoptosis through the SIRT1-FoxO1 pathway [J].

Chen, Chun-Juan ;

Yu, Wei ;

Fu, Yu-Cai ;

Wang, Xin ;

Li, Ji-Lin ;

Wang, Wei .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2009, 378 (03) :389-393

[9]

Early inhibition of HIF-1α with small interfering RNA reduces ischemic-reperfused brain injury in rats [J].

Chen, Chunhua ;

Hu, Qin ;

Yan, Junhao ;

Yang, Xiaomei ;

Shi, Xianzhong ;

Lei, Jiliang ;

Chen, Lin ;

Huang, Hongyun ;

Han, Jingyan ;

Zhang, John H. ;

Zhou, Changman .

NEUROBIOLOGY OF DISEASE, 2009, 33 (03) :509-517

[10]

Silent information regulator 2 potentiates Foxo1-mediated transcription through its deacetylase activity [J].

Daitoku, H ;

Hatta, M ;

Matsuzaki, H ;

Aratani, S ;

Ohshima, T ;

Miyagishi, M ;

Nakajima, T ;

Fukamizu, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (27) :10042-10047

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