Site-specific Srb10-dependent phosphorylation of the yeast mediator subunit Med2 regulates gene expression from the 2-μm plasmid

被引:30
作者
Hallberg, M
Polozkov, GV
Hu, GZ
Beve, J
Gustafsson, CM
Ronne, H
Björklund, S
机构
[1] Umea Univ, Dept Med Biochem & Biophys, SE-90187 Umea, Sweden
[2] Swedish Univ Agr Sci, Dept Plant Biol & Forest Genet, SE-75007 Uppsala, Sweden
[3] Karolinska Inst, Novum, Dept Med Nutr, S-14186 Huddinge, Sweden
关键词
D O I
10.1073/pnas.0400221101
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The yeast Mediator complex is required for transcriptional regulation both in vivo and in vitro, and its function is conserved in all eukaryotes. Mediator interacts with both transcriptional activators and RNA polymerase II, but little is known about the mechanisms by which it operates at the molecular level. Here, we show that the cyclin-dependent kinase Srb10 interacts with, and phosphorylates, the Med2 subunit of Mediator both in vivo and in vitro. A point mutation of the single phosphorylation site in Med2 results in a strongly reduced expression of the REP1, REP2, FLP1, and RAF1 genes, which are all located on the endogenous 2-mum plasmid. Combined with previous studies on the effects of SRB10/SRB11 deletions, our data suggest that posttranslational modifications of Mediator subunits are important for regulation of gene expression.
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页码:3370 / 3375
页数:6
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