Exosomes from C2C12 myoblasts enhance osteogenic differentiation of MC3T3-E1 pre-osteoblasts by delivering miR-27a-3p

被引：112

作者：

Xu, Qian ^{[1
,2
]}

Cui, Yazhou ^{[2
]}

Luan, Jing ^{[2
]}

Zhou, Xiaoyan ^{[1
,2
]}

Li, Haiying ^{[1
,2
]}

Han, Jinxiang ^{[1
,2
]}

机构：

[1] Univ Jinan, Sch Med & Life Sci, Shandong Acad Med Sci, Jinan, Shandong, Peoples R China

[2] Shandong Acad Med Sci, Shandong Med Biotechnol Ctr, Key Lab Rare Dis Res Shandong Prov, Jinan, Shandong, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 498卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Exosome; Muscle-bone interaction; Osteoblast; microRNA; MATRIX VESICLES; COMMUNICATION; MUSCLE; CELL; BONE;

D O I：

10.1016/j.bbrc.2018.02.144

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Many regulators have been identified to participate in the cross-talk between muscle and bone, however, most previous studies focus on secreting proteins. In this study, we demonstrated that exosomes from myoblasts C2C12 can promote pre-osteoblasts MC3T3-E1 differentiation to osteoblasts. We revealed that the effect of C2C12 exosomes depended on its miR-27a-3p component, they can increase miR-27a-3p level in the recipient cells, and decrease its direct target adenomatous polyposis coli (APC) expression, thus activating P-catenin pathway. Furthermore, C2C12 exosomes failed to exert above effects when miR-27a-3p was deprived. These findings indicates exosomal microRNAs can be regarded as a novel type of "myokines" with osteogenesis promoting potential, which would broad our understanding of the muscle-bone interaction under physiological and pathological conditions. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：32 / 37

页数：6

共 17 条

[1]

Bone and muscle: Interactions beyond mechanical [J].