MicroRNAs in Plasma and Cerebrospinal Fluid as Potential Markers for Alzheimer's Disease

被引：212

作者：

Kiko, Takehiro ^{[1
]}

Nakagawa, Kiyotaka ^{[1
]}

Tsuduki, Tsuyoshi ^{[2
]}

Furukawa, Katsutoshi ^{[3
]}

Arai, Hiroyuki ^{[3
]}

Miyazawa, Teruo ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Food & Biodynam Chem Lab, Sendai, Miyagi 9818555, Japan

[2] Tohoku Univ, Grad Sch Agr Sci, Lab Food & Biomol Sci, Sendai, Miyagi 9818555, Japan

[3] Tohoku Univ, Inst Dev Aging & Canc, Inst Gerontol & Geriatr, Sendai, Miyagi 9818555, Japan

来源：

JOURNAL OF ALZHEIMERS DISEASE | 2014年 / 39卷 / 02期

关键词：

Alzheimer's disease; cerebrospinal fluid; miRNA; plasma; AMYLOID-BETA; CIRCULATING MICRORNAS; EXPRESSION; BIOMARKERS; BRAIN; CANCER; DIAGNOSIS; OVEREXPRESSION; PROGRESSION; METABOLISM;

D O I：

10.3233/JAD-130932

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The development of Alzheimer's disease (AD) biomarkers remains an unmet challenge, and new approaches that can improve current AD biomarker strategies are needed. Recent reports suggested that microRNA (miRNA) profiling of biological fluids has emerged as a diagnostic tool for several pathologic conditions. In this study, we measured six candidate miRNAs (miR-9, miR-29a, miR-29b, miR-34a, miR-125b, and miR-146a) in plasma and cerebrospinal fluid (CSF) of AD and normal subjects by using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) to evaluate their potential usability as AD biomarkers. The qRT-PCR results showed that plasma miR-34a and miR-146a levels, and CSF miR-34a, miR-125b, and miR-146a levels in AD patients were significantly lower than in control subjects. On the other hand, CSF miR-29a and miR-29b levels were significantly higher than in control subjects. Our results provide a possibility that miRNAs detected in plasma and CSF can serve as biomarkers for AD.

引用

页码：253 / 259

页数：7

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