Immobilization of catalase on cotton fabric oxidized by sodium periodate

被引：22

作者：

Wang, Qiang ^{[1
]}

Li, Cheng Xuan ^{[1
]}

Fan, Xuerong ^{[1
]}

Wang, Ping ^{[1
]}

Cui, Li ^{[1
]}

机构：

[1] Jiangnan Univ, Coll Text & Clothing, Key Lab Sci & Technol Eco Text, Minist Educ, Wuxi 214122, Peoples R China

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 2008年 / 26卷 / 05期

基金：

国家高技术研究发展计划(863计划);

关键词：

Catalase; immobilization; oxidized cotton fabric; sodium periodate;

D O I：

10.1080/10242420802335031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cotton fabric was first oxidized with sodium periodate, and then employed to immobilize catalase. Optimization studies for oxidation of the fabric and immobilization of the enzyme were performed. The properties of the immobilized catalase were examined and compared with those of the free enzyme. A high activity of the immobilized enzyme was obtained when the fabric was oxidized at 40 degrees C and pH 6.0 for 8h in a bath containing 0.20 mol L-1 sodium periodate and the enzyme was immobilized at 4 degrees C for 24h with a catalase dosage of 120.0 U mL(-1). The immobilized enzyme exhibited optimum activity at 40 degrees C, while the free enzyme had optimal temperature of 30 degrees C, suggesting that the immobilized catalase could be used in a broader temperature range. Both the immobilized and free enzyme had pH optima of 7.0. The staining test and reusability showed that the catalase was fixed covalently on the oxidized cotton fabric.

引用

页码：437 / 443

页数：7

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