Immobilization of catalases from Bacillus SF on alumina for the treatment of textile bleaching effluents

被引：102

作者：

Costa, SA

Tzanov, T

Paar, A

Gudelj, M

Gübitz, GM

Cavaco-Paulo, A ^{[1
]}

机构：

[1] Univ Minho, Dept Text Engn, P-4800 Guimaraes, Portugal

[2] Graz Univ Technol, Dept Environm Biotechnol, A-8010 Graz, Austria

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2001年 / 28卷 / 9-10期

关键词：

catalase; covalent immobilization; thermo- and alkaline stability; hydrogen peroxide;

D O I：

10.1016/S0141-0229(01)00335-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A catalase preparation from a newly isolated Bacillus sp. was covalently immobilized on silanized alumina using glutaraldehyde as crosslinking agent. The effect of the coupling time of the enzyme-support reaction was determined in terms of protein recovery and immobilization yield and a certain balance point was found after which the activity recovery decreased. The activity profile of the immobilized catalase at high pH and temperature was investigated. The immobilized enzyme showed higher stabilities (214 h at pH 11, 30 degreesC) at alkaline pH than the free enzyme (10 h at pH 11, 30 degreesC), The immobilized catalase was inhibited by anionic stabilizers os surfactants added to the hydrogen peroxide substrate solution. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：815 / 819

页数：5

共 18 条

[1] Aebi H. E., 1974, METHOD ENZYMAT AN, P672
[2] [Anonymous], APPL BIOCH BIOTECHNO
[3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4] THE EFFECT OF AQUEOUS SURFACTANT SOLUTIONS ON ALCOHOL-DEHYDROGENASE (LADH)
CREAGH, AL
PRAUSNITZ, JM
BLANCH, HW
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1993, 41 (01) : 156 - 161
[5] Emerson D, 1996, BIOTECHNOL TECH, V10, P673
[6] On the use of chitosan-immobilized β-glucosidase in wine-making:: kinetics and enzyme inhibition
Gallifuoco, A
D'Ercole, L
Alfani, F
Cantarella, M
Spagna, G
Pifferi, PG
[J]. PROCESS BIOCHEMISTRY, 1998, 33 (02) : 163 - 168
[7] IMMOBILIZATION STABILIZATION OF ALPHA-CHYMOTRYPSIN BY COVALENT ATTACHMENT TO ALDEHYDE AGAROSE GELS
GUISAN, JM
BASTIDA, A
CUESTA, C
FERNANDEZLAFUENTE, R
ROSELL, CM
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (10) : 1144 - 1152
[8] IMMOBILIZATION OF CATALASE BY POLYION COMPLEX
KOHJIYA, S
MAEDA, K
YAMASHITA, S
[J]. JOURNAL OF APPLIED POLYMER SCIENCE, 1991, 43 (12) : 2219 - 2222
[9] MUELLER S, 1997, ANAL BIOCHEM, V245, P50
[10] PAAR A, IN PRESS J BIOTECHNO

← 1 2 →