Biosensors using the Si nanochannel junction-isolated from the Si bulk substrate

被引:4
作者
Ahn, Chang-Geun [1 ]
Park, Chan Woo [1 ]
Yang, Jong-Heon [1 ]
Ah, Chil Seong [1 ]
Kim, Ansoon [1 ]
Kim, Tae-Youb [1 ]
Yu, Han Young [2 ]
Jang, Moongyu [2 ]
Kim, Sang-Hoon [2 ]
Baek, In-Bok [3 ,4 ]
Lee, Seongjae [3 ,4 ]
Sung, Gun Yong [1 ]
机构
[1] Elect & Telecommun Res Inst, Dept BT Convergence Technol Res, Taejon 305700, South Korea
[2] Elect & Telecommun Res Inst, IT Covergence & Components Lab, Taejon 305700, South Korea
[3] Hanyang Univ, Quantum Photon Sci Res Ctr, Seoul 133791, South Korea
[4] Hanyang Univ, Res Inst Nat Sci, Seoul 133791, South Korea
关键词
atomic force microscopy; biomolecular electronics; biosensors; DNA; elemental semiconductors; field effect transistors; gold; leakage currents; nanobiotechnology; nanoelectronics; nanofabrication; nanoparticles; nanosensors; p-n junctions; silicon; NANOWIRES; ARRAYS;
D O I
10.1063/1.3266161
中图分类号
O59 [应用物理学];
学科分类号
摘要
The biosensor using a silicon nanochannel field effect transistor has been developed on the basis of a bulk silicon substrate instead of an expensive silicon-on-insulator material, for low cost applications. "Top-down" fabricated Si nanochannels for detecting prostate specific antigen (PSA) are electrically isolated from the bulk Si substrate only by a reverse-biased p-n junction with very low reverse leakage currents. The reverse leakage current flowing through the p-n junction is small enough to be ignored, when compared with the current flowing through the p-type Si channel; roughly 100 times lower. The surface immobilization of anti-PSA has been confirmed by the specific binding test of DNA stabilized Au nanoparticles (NPs), showing 1200 NPs/mu m(2) and atomic force microscopy surface analysis. The injection of 10 ng/ml PSA solutions leads to a clear increase in the conductance of Si nanochannels, indicating the specific binding of PSA with the immobilized anti-PSA. The similar signal changes were observed on the injection of the 1 pg/ml PSA, very low concentration of PSA.
引用
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页数:5
相关论文
共 12 条
[1]   Electrochemically programmed, spatially selective biofunctionalization of silicon wires [J].
Bunimovich, YL ;
Ge, GL ;
Beverly, KC ;
Ries, RS ;
Hood, L ;
Heath, JR .
LANGMUIR, 2004, 20 (24) :10630-10638
[2]   Selective functionalization of In2O3 nanowire mat devices for biosensing applications [J].
Curreli, M ;
Li, C ;
Sun, YH ;
Lei, B ;
Gundersen, MA ;
Thompson, ME ;
Zhou, CW .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (19) :6922-6923
[3]   Ultrasensitive, label-free, and real-time immunodetection using silicon field-effect transistors [J].
Kim, Ansoon ;
Ah, Chil Seong ;
Yu, Han Young ;
Yang, Jong-Heon ;
Baek, In-Bok ;
Ahn, Chang-Guen ;
Park, Chan Woo ;
Jun, Myung Sim ;
Lee, Sungjae .
APPLIED PHYSICS LETTERS, 2007, 91 (10)
[4]  
Law M, 2002, ANGEW CHEM INT EDIT, V41, P2405, DOI 10.1002/1521-3773(20020703)41:13<2405::AID-ANIE2405>3.0.CO
[5]  
2-3
[6]   Complementary detection of prostate-specific antigen using ln2O3 nanowires and carbon nanotubes [J].
Li, C ;
Curreli, M ;
Lin, H ;
Lei, B ;
Ishikawa, FN ;
Datar, R ;
Cote, RJ ;
Thompson, ME ;
Zhou, CW .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (36) :12484-12485
[7]   Sequence-specific label-free DNA sensors based on silicon nanowires [J].
Li, Z ;
Chen, Y ;
Li, X ;
Kamins, TI ;
Nauka, K ;
Williams, RS .
NANO LETTERS, 2004, 4 (02) :245-247
[8]   Preparation of nucleic acid functionalized carbon nanotube Arrays [J].
Nguyen, CV ;
Delzeit, L ;
Cassell, AM ;
Li, J ;
Han, J ;
Meyyappan, M .
NANO LETTERS, 2002, 2 (10) :1079-1081
[9]   Recent advances in biologically sensitive field-effect transistors (BioFETs) [J].
Schöning, MJ ;
Poghossian, A .
ANALYST, 2002, 127 (09) :1137-1151
[10]   Label-free immunodetection with CMOS-compatible semiconducting nanowires [J].
Stern, Eric ;
Klemic, James F. ;
Routenberg, David A. ;
Wyrembak, Pauline N. ;
Turner-Evans, Daniel B. ;
Hamilton, Andrew D. ;
LaVan, David A. ;
Fahmy, Tarek M. ;
Reed, Mark A. .
NATURE, 2007, 445 (7127) :519-522