Strong and Reversible Monovalent Supramolecular Protein Immobilization

被引：69

作者：

Young, Jacqui F. ^{[2
,3
]}

Nguyen, Hoang D. ^{[2
,3
]}

Yang, Lanti ^{[1
]}

Huskens, Jurriaan ^{[1
]}

Jonkheijm, Pascal ^{[1
]}

Brunsveld, Luc ^{[2
,3
]}

机构：

[1] Univ Twente, Lab Mol Nonofabricat, MESA Inst Nanotechnol, NL-7500 AE Enschede, Netherlands

[2] Max Planck Soc, Chem Genom Ctr, D-44227 Dortmund, Germany

[3] Eindhoven Univ Technol, Dept Biomed Engn, Biol Chem Lab, NL-5612 AZ Eindhoven, Netherlands

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 02期

关键词：

cucurbituril; host-guest systems; protein immobilization; protein ligation; supramolecular chemistry; FLUORESCENT PROTEIN; BETA-CYCLODEXTRIN; CHEMISTRY; HOST; RECOGNITION; FERROCENE; COMPLEXES; BINDING;

D O I：

10.1002/cbic.200900599

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins with an iron clasp: Site-selective incorporation of a ferrocene molecule into a protein allows for easy, strong, and reversible supramolecular protein immobilization through a selective monovalent interaction of the ferrocene with a cucurbit[7]uril immobilized on a gold surface. The proteins can be printed in a uniform monolayer that is resistant to washing conditions, but can be removed through competition with an excess of ferrocene ligand. (Figure Presented). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：180 / 183

页数：4

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