Strong and Reversible Monovalent Supramolecular Protein Immobilization

被引：69

作者：

Young, Jacqui F. ^{[2
,3
]}

Nguyen, Hoang D. ^{[2
,3
]}

Yang, Lanti ^{[1
]}

Huskens, Jurriaan ^{[1
]}

Jonkheijm, Pascal ^{[1
]}

Brunsveld, Luc ^{[2
,3
]}

机构：

[1] Univ Twente, Lab Mol Nonofabricat, MESA Inst Nanotechnol, NL-7500 AE Enschede, Netherlands

[2] Max Planck Soc, Chem Genom Ctr, D-44227 Dortmund, Germany

[3] Eindhoven Univ Technol, Dept Biomed Engn, Biol Chem Lab, NL-5612 AZ Eindhoven, Netherlands

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 02期

关键词：

cucurbituril; host-guest systems; protein immobilization; protein ligation; supramolecular chemistry; FLUORESCENT PROTEIN; BETA-CYCLODEXTRIN; CHEMISTRY; HOST; RECOGNITION; FERROCENE; COMPLEXES; BINDING;

D O I：

10.1002/cbic.200900599

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins with an iron clasp: Site-selective incorporation of a ferrocene molecule into a protein allows for easy, strong, and reversible supramolecular protein immobilization through a selective monovalent interaction of the ferrocene with a cucurbit[7]uril immobilized on a gold surface. The proteins can be printed in a uniform monolayer that is resistant to washing conditions, but can be removed through competition with an excess of ferrocene ligand. (Figure Presented). © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：180 / 183

页数：4

共 32 条

[21] Unusual electrochemical properties of the inclusion complexes of ferrocenium and cobaltocenium with cucurbit[7]uril [J].

Ong, W ;

Kaifer, AE .

ORGANOMETALLICS, 2003, 22 (21) :4181-4183

[22] Supramolecular chemistry in water [J].

Oshovsky, Gennady V. ;

Reinhoudt, David N. ;

Verboom, Willem .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (14) :2366-2393

[23] Sequence recognition and self-sorting of a dipeptide by cucurbit[6]uril and cucurbit[7]uril [J].

Rekharsky, Mikhail V. ;

Yamamura, Hatsuo ;

Ko, Young Ho ;

Selvapalam, N. ;

Kim, Kimoon ;

Inoue, Yoshihisa .

CHEMICAL COMMUNICATIONS, 2008, (19) :2236-2238

[24] Supramolecular control of split-GFP reassembly by conjugation of β-cyclodextrin and coumarin units [J].

Sakamoto, Seiji ;

Kudo, Kazuaki .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (29) :9574-9582

[25] Advances in fluorescent protein technology [J].

Shaner, Nathan C. ;

Patterson, George H. ;

Davidson, Michael W. .

JOURNAL OF CELL SCIENCE, 2007, 120 (24) :4247-4260

[26]

Smiljanic N., 2006, ANGEW CHEM, V118, P5591

[27] Supramolecular control of oligosaccharide-protein interactions: Switchable and tunable ligands for concanavalin A based on cyclodextrin [J].

Smiljanic, Nicolas ;

Moreau, Vincent ;

Yockot, Duplex ;

Benito, Juan M. ;

Fernandez, Jose M. Garcia ;

Djedaini-Pilard, Florence .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (33) :5465-5468

[28] Modulation of Protein Dimerization by a Supramolecular Host-Guest System [J].

Uhlenheuer, Dana A. ;

Wasserberg, Dorothee ;

Nguyen, Hoang ;

Zhang, Li ;

Blum, Christian ;

Subramaniam, Vinod ;

Brunsveld, Luc .

CHEMISTRY-A EUROPEAN JOURNAL, 2009, 15 (35) :8779-8790

[29] Crystallographic and energetic analysis of binding of selected anions to the yellow variants of green fluorescent protein [J].

Wachter, RM ;

Yarbrough, D ;

Kallio, K ;

Remington, SJ .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 301 (01) :157-171

[30] HREELS and XPS studies of ferrocene on Ag(100) [J].

Woodbridge, CM ;

Pugmire, DL ;

Johnson, RC ;

Boag, NM ;

Langell, MA .

JOURNAL OF PHYSICAL CHEMISTRY B, 2000, 104 (14) :3085-3093

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