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Growth and sporulation of Bacillus cereus ATCC 14579 under defined conditions:: Temporal expression of genes for key sigma factors

被引：65
作者：
de Vries, YP
Hornstra, LM
de Vos, WM
Abee, T
机构：
[1] Wageningen Ctr Food Sci, NL-6700 AN Wageningen, Netherlands
[2] Wageningen UR, Lab Food Microbiol, Wageningen, Netherlands
[3] Agrotechnol & Food Innovat A&F, Wageningen, Netherlands
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 04期
关键词：
D O I：
10.1128/AEM.70.4.2514-2519.2004
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An airlift fermentor system allowing precise regulation of pH and aeration combined with a chemically defined medium was used to study growth and sporulation of Bacillus cereus ATCC 14579. Sporulation was complete and synchronous. Expression of sigA, sigB, sigF, and sigG was monitored with real-time reverse transcription-PCR, and the pattern qualitatively resembled that of Bacillus subtilis. This method allows reproducible production of stable spores, while the synchronous growth and defined conditions are excellently suitable for further gene expression studies of cellular differentiation of B. cereus.
引用
收藏
页码：2514 / 2519
页数：6
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