An N-terminal method for peptide solubilisation

被引：14

作者：

Englebretsen, DR ^{[1
]}

Robillard, GT ^{[1
]}

机构：

[1] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Biochem, NL-9747 AG Groningen, Netherlands

来源：

TETRAHEDRON | 1999年 / 55卷 / 21期

关键词：

peptides and polypeptides; solid phase synthesis; cleavage reactions;

D O I：

10.1016/S0040-4020(99)00309-9

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Peptide-constructs of the form (solubilising peptide)-[NH(CH2)(2)SO2(CH2)(2)O-CO]-(insoluble peptide) were synthesised by Boc SPPS. The HPLC-purified peptide-constructs were cleaved with aqueous base to liberate the insoluble peptides as precipitates. (C) 1999 Published by Elsevier Science Ltd. All rights reserved.

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页码：6623 / 6634

页数：12

相关论文

共 12 条

[1] Synthesis of a versatile purification handle for use with Boc chemistry solid phase peptide synthesis [J].

Canne, LE ;

Winston, RL ;

Kent, SBH .

TETRAHEDRON LETTERS, 1997, 38 (19) :3361-3364

[2] Synthesis of hydrophobic peptides: An Fmoc "Solubilising Tail" method [J].

Choma, CT ;

Robillard, GT ;

Englebretsen, DR .

TETRAHEDRON LETTERS, 1998, 39 (16) :2417-2420

[3] A NOVEL THIOETHER LINKER - CHEMICAL SYNTHESIS OF A HIV-1 PROTEASE ANALOG BY THIOETHER LIGATION [J].

ENGLEBRETSEN, DR ;

GARNHAM, BG ;

BERGMAN, DA ;

ALEWOOD, PF .

TETRAHEDRON LETTERS, 1995, 36 (48) :8871-8874

[4] Synthesis of a designed transmembrane protein by thioether ligation of solubilised segments:: Nα-haloacetylated peptides survived resin cleavage using TFA with EDT as scavenger [J].

Englebretsen, DR ;

Choma, CT ;

Robillard, GT .

TETRAHEDRON LETTERS, 1998, 39 (27) :4929-4932

[5] Boc SPPS of two hydrophobic peptides using a ''solubilising tail'' strategy: Dodecaalanine and chemotactic protein 10(42-55) [J].

Englebretsen, DR ;

Alewood, PF .

TETRAHEDRON LETTERS, 1996, 37 (46) :8431-8434

[6] CHEMOSELECTIVE ONE-STEP PURIFICATION METHOD FOR PEPTIDES SYNTHESIZED BY THE SOLID-PHASE TECHNIQUE [J].

FUNAKOSHI, S ;

FUKUDA, H ;

FUJII, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :6981-6985

[7]

LACKMAN M, 1993, J IMMUNOL, V7, P2981

[8] SYNTHESIS OF SULFUR ANALOGS OF BATYL AND CHIMYL ALCOHOLS [J].

LAWSON, DD ;

GETZ, HR ;

MILLER, DA .

JOURNAL OF ORGANIC CHEMISTRY, 1961, 26 (02) :615-&

[9] Simple procedure for reversed-phase high-performance liquid chromatographic purification of long hydrophobic peptides that form transmembrane helices [J].

Lew, S ;

London, E .

ANALYTICAL BIOCHEMISTRY, 1997, 251 (01) :113-116

[10]

SCHNOLZER M, 1992, INT J PEPT PROT RES, V40, P180