Synthesis of a versatile purification handle for use with Boc chemistry solid phase peptide synthesis

被引:26
作者
Canne, LE [1 ]
Winston, RL [1 ]
Kent, SBH [1 ]
机构
[1] SCRIPPS RES INST, LA JOLLA, CA 92037 USA
关键词
D O I
10.1016/S0040-4039(97)00651-5
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
The synthesis of a versatile handle for the purification of synthetic peptides is described. The Boc-aminoethylsulfonylethyloxycarbonyl handle is coupled to the NH2-terminus of the resin-bound peptide. Removal of the Boc group affords a free amine which can be derivatized with any of a variety of functionalities. Cleavage from the resin gives a peptide functionalized for covalent chemoselective reaction with the column support. Desired full length peptide is eluted from the column by cleavage of the handle by treatment with base. (C) 1997 Elsevier Science Ltd.
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页码:3361 / 3364
页数:4
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