Architecture of the major component of the type III secretion system export apparatus

被引：175

作者：

Abrusci, Patrizia ^{[1
]}

Vergara-Irigaray, Marta ^{[1
,2
]}

Johnson, Steven ^{[1
]}

Beeby, Morgan D. ^{[3
,4
]}

Hendrixson, David R. ^{[5
]}

Roversi, Pietro ^{[1
]}

Friede, Miriam E. ^{[1
]}

Deane, Janet E. ^{[1
]}

Jensen, Grant J. ^{[3
,4
]}

Tang, Christoph M. ^{[1
]}

Lea, Susan M. ^{[1
]}

机构：

[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England

[2] Univ London Imperial Coll Sci Technol & Med, Ctr Mol Microbiol & Infect, London, England

[3] CALTECH, Dept Biol, Pasadena, CA 91125 USA

[4] CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USA

[5] Univ Texas SW Med Ctr Dallas, Dept Microbiol, Dallas, TX 75390 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2013年 / 20卷 / 01期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

FLAGELLAR PROTEIN EXPORT; PROTON MOTIVE FORCE; CYTOPLASMIC DOMAIN; ESCHERICHIA-COLI; CRYOELECTRON TOMOGRAPHY; BACTERIAL FLAGELLA; MEMBRANE-PROTEINS; NEEDLE COMPLEX; FLII ATPASE; SALMONELLA;

D O I：

10.1038/nsmb.2452

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Type III secretion systems (T3SSs) are bacterial membrane-embedded nanomachines designed to export specifically targeted proteins from the bacterial cytoplasm. Secretion through T3SS is governed by a subset of inner membrane proteins termed the 'export apparatus'. We show that a key member of the Shigella flexneri export apparatus, MxiA, assembles into a ring essential for secretion in vivo. The ring-forming interfaces are well-conserved in both nonflagellar and flagellar homologs, implying that the ring is an evolutionarily conserved feature in these systems. Electron cryo-tomography revealed a T3SS-associated cytoplasmic torus of size and shape corresponding to those of the MxiA ring aligned to the secretion channel located between the secretion pore and the ATPase complex. This defines the molecular architecture of the dominant component of the export apparatus and allows us to propose a model for the molecular mechanisms controlling secretion.

引用

页码：99 / U126

页数：8

共 63 条

[1] Fast tomographic reconstruction on multicore computers [J].

Agulleiro, J. I. ;

Fernandez, J. J. .

BIOINFORMATICS, 2011, 27 (04) :582-583

[2] Chaperone release and unfolding of substrates in type III secretion [J].

Akeda, Y ;

Galán, JE .

NATURE, 2005, 437 (7060) :911-915

[3] MXIA OF SHIGELLA-FLEXNERI-2A, WHICH FACILITATES EXPORT OF INVASION PLASMID ANTIGENS, ENCODES A HOMOLOG OF THE LOW-CALCIUM-RESPONSE PROTEIN, LCRD, OF YERSINIA-PESTIS [J].

ANDREWS, GP ;

MAURELLI, AT .

INFECTION AND IMMUNITY, 1992, 60 (08) :3287-3295

[4] Shigella deploy multiple countermeasures against host innate immune responses [J].

Ashida, Hiroshi ;

Ogawa, Michinaga ;

Kim, Minsoo ;

Suzuki, Shiho ;

Sanada, Takahito ;

Punginelli, Claire ;

Mimuro, Hitomi ;

Sasakawa, Chihiro .

CURRENT OPINION IN MICROBIOLOGY, 2011, 14 (01) :16-23

[5] FlhA provides the adaptor for coordinated delivery of late flagella building blocks to the type III secretion system [J].

Bange, Gert ;

Kuemmerer, Nico ;

Engel, Christoph ;

Bozkurt, Gunes ;

Wild, Klemens ;

Sinning, Irmgard .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (25) :11295-11300

[6] The rotary motor of bacterial flagella [J].

Berg, HC .

ANNUAL REVIEW OF BIOCHEMISTRY, 2003, 72 :19-54

[7] Refinement of severely incomplete structures with maximum likelihood in BUSTER-TNT [J].

Blanc, E ;

Roversi, P ;

Vonrhein, C ;

Flensburg, C ;

Lea, SM ;

Bricogne, G .

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 2004, 60 :2210-2221

[8] Structure and composition of the Shigella flexneri 'needle complex', a part of its type III secreton [J].

Blocker, A ;

Jouihri, N ;

Larquet, E ;

Gounon, P ;

Ebel, F ;

Parsot, C ;

Sansonetti, P ;

Allaoui, A .

MOLECULAR MICROBIOLOGY, 2001, 39 (03) :652-663

[9] What's the point of the type III secretion system needle? [J].

Blocker, Ariel J. ;

Deane, Janet E. ;

Veenendaal, Andreas K. J. ;

Roversi, Pietro ;

Hodgkinson, Julie L. ;

Johnson, Steven ;

Lea, Susan M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (18) :6507-6513

[10] Structural diversity of bacterial flagellar motors [J].

Chen, Songye ;

Beeby, Morgan ;

Murphy, Gavin E. ;

Leadbetter, Jared R. ;

Hendrixson, David R. ;

Briegel, Ariane ;

Li, Zhuo ;

Shi, Jian ;

Tocheva, Elitza I. ;

Mueller, Axel ;

Dobro, Megan J. ;

Jensen, Grant J. .

EMBO JOURNAL, 2011, 30 (14) :2972-2981

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