A European field strain of bluetongue virus derived from two parental vaccine strains by genome segment reassortment

被引：116

作者：

Batten, Carrie A. ^{[1
]}

Maan, Sushila ^{[1
]}

Shaw, Andrew E. ^{[1
]}

Maan, Narender S. ^{[1
]}

Mertens, Peter P. C. ^{[1
]}

机构：

[1] Inst Anim Hlth, Arbovirus Res Grp, Pirbright GU24 0NF, England

来源：

VIRUS RESEARCH | 2008年 / 137卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

bluetongue; BTV; orbivirus; dsRNA; reassortment; BTV vaccine;

D O I：

10.1016/j.virusres.2008.05.016

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Since 1998, nine bluetongue virus (BTV) strains from serotypes 1, 2. 4, 8, 9 and 16 have invaded Europe, killing >2 million animals (mainly sheep). Live vaccine strains of BTV-2, 4, 9 and 16 have also been used in the region. The BTV genome is composed of ten linear-segments of dsRNA, and events in Europe have provided opportunities for different strains to exchange/reassort genome segments, generating novel progeny-vi ruses. Genome segment 2 (Seg-2) encodes outer capsid protein VP2, the primary determinant of virus serotype, while Seg-5 encodes NS1, which forms 'tubules' within the cell-cytoplasm. Seg-2 and Seg-5 from 15 European isolates, and vaccine/reference strains, of BTV-2 and BTV-16, were sequenced. Isolates from the same serotype showed >92% nt identity in Seg-5, but <84% identity between types. However, published data for Seg-5 of BTV-16 from Italy 2002 showed <83% nt identity with other BTV-16 strains, but was identical to the BTV-2 vaccine strain (used in Italy during 2002, and annually in a multivalent vaccine in Israel since 1995) indicating that ITL2002 is a reassortant between the BTV-2 and BTV-16 vaccine strains. This represents the first detection of a reassortant BTV strain within Europe, highlighting concerns about the use of live BTV vaccines in the region. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：56 / 63

页数：8

共 61 条

[1] Molecular differentiation between NS1 gene of a field strain Bluetongue virus serotype 2 (BTV-2) and NS1 gene of an attenuated BTV-2 vaccine [J].

Agüero, M ;

Arias, M ;

Romero, LJ ;

Zamora, MJ ;

Sánchez-Vizcaíno, JM .

VETERINARY MICROBIOLOGY, 2002, 86 (04) :337-341

[2] A duplex RT-PCR assay for detection of genome segment 7 (VP7 gene) from 24 BTV serotypes [J].

Anthony, S. ;

Jones, H. ;

Darpel, K. E. ;

Elliott, H. ;

Maan, S. ;

Samuel, A. ;

Mellor, P. S. ;

Mertens, P. P. C. .

JOURNAL OF VIROLOGICAL METHODS, 2007, 141 (02) :188-197

[3] Strategies for the sequence determination of viral dsRNA genomes [J].

Attoui, H ;

Billoir, F ;

Cantaloube, JF ;

Biagini, P ;

de Micco, P ;

de Lamballerie, X .

JOURNAL OF VIROLOGICAL METHODS, 2000, 89 (1-2) :147-158

[4] The NS3 proteins of global strains of bluetongue virus evolve into regional topotypes through negative (purifying) selection [J].

Balasuriya, U. B. R. ;

Nadler, S. A. ;

Wilson, W. C. ;

Pritchard, L. I. ;

Smythe, A. B. ;

Savini, G. ;

Monaco, F. ;

De Santis, P. ;

Zhang, N. ;

Tabachnick, W. J. ;

MacLachlan, N. J. .

VETERINARY MICROBIOLOGY, 2008, 126 (1-3) :91-100

[5] Molecular epidemiology of bluetongue virus in Portugal during 2004-2006 outbreak [J].

Barros, Silvia C. ;

Ramos, Fernanda ;

Luis, Tiago M. ;

Vaz, Andreia ;

Duarte, Margarida ;

Henriques, Margarida ;

Cruz, Benedita ;

Fevereiro, Miguel .

VETERINARY MICROBIOLOGY, 2007, 124 (1-2) :25-34

[6] Phylogenetic analysis of the S7 gene does not segregate Chinese strains of bluetongue virus into a single topotype [J].

Bonneau, KR ;

Zhang, NZ ;

Wilson, WC ;

Zhu, JB ;

Zhang, FQ ;

Li, ZH ;

Zhang, KL ;

Xiao, L ;

Xiang, WB ;

MacLachlan, NJ .

ARCHIVES OF VIROLOGY, 2000, 145 (06) :1163-1171

[7] The epidemiology and diagnosis of bluetongue with particular reference to Corsica [J].

Breard, E ;

Hamblin, C ;

Hammoumi, S ;

Sailleau, C ;

Dauphin, G ;

Zientara, S .

RESEARCH IN VETERINARY SCIENCE, 2004, 77 (01) :1-8

[8] CROSS-NEUTRALIZATION OF GENETIC REASSORTANTS OF BLUETONGUE VIRUS SEROTYPES 20 AND 21 [J].

COWLEY, JA ;

GORMAN, BM .

VETERINARY MICROBIOLOGY, 1989, 19 (01) :37-51

[9] Clinical signs and pathology shown by British sheep and cattle infected with bluetongue virus serotype 8 derived from the 2006 outbreak in northern Europe [J].

Darpel, K. E. ;

Batten, C. A. ;

Veronesi, E. ;

Shaw, A. E. ;

Anthony, S. ;

Bachanek-Bankowska, K. ;

Kgosana, L. ;

Bin-Tarif, A. ;

Carpenter, S. ;

Mueller-Doblies, U. U. ;

Takamatsu, H-H. ;

Mellor, R. S. ;

Mertens, P. R. C. ;

Oura, C. A. L. .

VETERINARY RECORD, 2007, 161 (08) :253-261

[10]

DEMATTOS CCP, 1991, AM J VET RES, V52, P1794

← 1 2 3 4 5 6 7 →