X-ray structure of a two-domain type laccase: A missing link in the evolution of multi-copper proteins

被引：41

作者：

Komori, Hirofumi ^{[1
,2
,3
]}

Miyazaki, Kentaro ^{[4
,5
]}

Higuchi, Yoshiki ^{[1
,2
,3
]}

机构：

[1] Hyogo Med Univ, Grad Sch Life Sci, Dept Life Sci, Kamigori, Hyogo 6781297, Japan

[2] Himeji Inst Technol, Kamigori, Hyogo 6781297, Japan

[3] RIKEN, SPring Ctr 8, Mikazuki, Hyogo 6795248, Japan

[4] Natl Inst Adv Ind Sci & Technol, Inst Biol Resources & Funct, Tsukuba, Ibaraki 3058566, Japan

[5] Univ Tokyo, Grad Sch Frontier Sci, Dept Med Genome Sci, Tsukuba, Ibaraki 3058566, Japan

来源：

FEBS LETTERS | 2009年 / 583卷 / 07期

基金：

日本学术振兴会;

关键词：

Crystal structure; Laccase; Metagenome; Molecular evolution; Multi-copper protein; Nitrite reductase; STREPTOMYCES-COELICOLOR; NITROSOMONAS-EUROPAEA; DIFFRACTION ANALYSIS; PHENOL OXIDASE; NITRITE; CRYSTALLIZATION; TOLERANCE; REDUCTASE; GENES; SITES;

D O I：

10.1016/j.febslet.2009.03.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A multi-copper protein with two cupredoxin-like domains was identified from our in-house metagenomic database. The recombinant protein, mgLAC, contained four copper ions/subunits, oxidized various phenolic and non-phenolic substrates, and had spectroscopic properties similar to common laccases. X-ray structure analysis revealed a homotrimeric architecture for this enzyme, which resembles nitrite reductase (NIR). However, a difference in copper coordination was found at the domain interface. mgLAC contains a T2/T3 tri-nuclear copper cluster at this site, whereas a mononuclear T2 copper occupies this position in NIR. The trimer is thus an essential part of the architecture of two-domain multi-copper proteins, and mgLAC may be an evolutionary precursor of NIR. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1189 / 1195

页数：7

共 32 条

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