Analysis of the dynamic organization of mixed protein fatty acid Langmuir films

被引:6
作者
Alexandre, S
Dubreuil, N
Fiol, C
Lair, D
Sommer, F
Duc, TM
Valleton, JM
机构
[1] BIOPHY RES SA,F-69000 VILLEURBANNE,FRANCE
[2] UNIV LYON,CENATS,F-69000 VILLEURBANNE,FRANCE
关键词
atomic force microscopy; Fourier transform infrared spectroscopy; interfaces; Langmuir-Blodgett films;
D O I
10.1016/S0040-6090(96)09115-8
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
The dynamic organization of a mixed Langmuir film of glucose oxidase and behenic acid has been studied. Samples of the mixed film transferred after different relaxation times were analyzed by UV-Vis and IR spectroscopies and by lateral force microscopy. The results demonstrate that the enzyme molecules present at the air/water interface are progressively covered by the behenic acid film. Moreover, we observe an increase of the quantity of glucose oxidase in the mixed film with time, showing that the adsorption of glucose oxidase under the behenic acid film continues during and after the relaxation process. (C) 1997 Elsevier Science S.A.
引用
收藏
页码:295 / 298
页数:4
相关论文
共 14 条
[1]   COMPARISON AT THE MICROSCOPIC SCALE OF MIXED FATTY-ACID PROTEIN LANGMUIR-BLODGETT-FILMS RESULTING FROM HORIZONTAL OR VERTICAL TRANSFER [J].
ALEXANDRE, S ;
DUBREUIL, N ;
FIOL, C ;
MALANDAIN, JJ ;
SOMMER, F ;
VALLETON, JM .
MICROSCOPY MICROANALYSIS MICROSTRUCTURES, 1994, 5 (4-6) :359-371
[2]   CONCENTRATION OF ENZYMES ADSORBED ONTO LANGMUIR FILMS AND CHARACTERISTICS OF A UREA SENSOR [J].
ARISAWA, S ;
ARISE, T ;
YAMAMOTO, R .
THIN SOLID FILMS, 1992, 209 (02) :259-263
[3]   CHARACTERIZATION OF ENZYMATIC STRUCTURES IN MIXED LANGMUIR-BLODGETT-FILMS BY SCANNING FORCE MICROSCOPY [J].
DUBREUIL, N ;
ALEXANDRE, S ;
FIOL, C ;
SOMMER, F ;
VALLETON, JM .
LANGMUIR, 1995, 11 (06) :2098-2102
[4]   Mixed Langmuir films of glucose oxidase and behenic acid: Structure and dynamics [J].
Dubreuil, N ;
Alexandre, S ;
Fiol, C ;
Valleton, JM .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1996, 181 (02) :393-398
[5]  
DUBRUEIL N, 1995, THESIS U ROUEN ROUEN
[6]   ELABORATION OF A GLUCOSE BIOSENSOR BASED ON LANGMUIR-BLODGETT TECHNOLOGY [J].
FIOL, C ;
VALLETON, JM ;
DELPIRE, N ;
BARBEY, G ;
BARRAUD, A ;
RUAUDELTEIXIER, A .
THIN SOLID FILMS, 1992, 210 (1-2) :489-491
[7]  
FIOL C, 1995, THIN SOLID FILMS, V261, P287
[8]   MOLECULAR RESOLUTION IMAGES OF ENZYME-CONTAINING LANGMUIR-BLODGETT-FILMS [J].
FIOL, C ;
ALEXANDRE, S ;
DELPIRE, N ;
VALLETON, JM ;
PARIS, E .
THIN SOLID FILMS, 1992, 215 (01) :88-93
[9]   LANGMUIR-BLODGETT FILMS AS CHEMICAL SENSORS [J].
MORIIZUMI, T .
THIN SOLID FILMS, 1988, 160 (1-2) :413-429
[10]   POSSIBLE APPLICATIONS FOR LANGMUIR-BLODGETT-FILMS [J].
PETTY, MC .
THIN SOLID FILMS, 1992, 210 (1-2) :417-426