Preliminary experiments for the analysis of tetrahydrotetrols of benz[a]anthracene and benzo[a]pyrene derived from their hemoglobin adducts using a coupled-column high-performance liquid chromatographic system

被引：4

作者：

Hackl, C

Lintelmann, J

Sauerbrey, R

Kettrup, A

机构：

[1] GSF FORSCHUNGSZENTRUM UNWELT & GESUNDHEIT GMBH,INST ONCOL CHEM,D-85758 OBERSCHLEISSHEIM,GERMANY

[2] E MERCK AG,D-64271 DARMSTADT,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 730卷 / 1-2期

关键词：

column switching; benz[a]anthracene; benzo[a]pyrene; polynuclear aromatic hydrocarbons; tetrahydrotetrols; haemoglobin;

D O I：

10.1016/0021-9673(95)01183-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Improved technologies for the detection of polycyclic aromatic hydrocarbon adducts are required for human biomonitoring. Therefore, a coupled-column high-performance liquid chromatographic method, with system-integrated sample processing, has been developed and its applicability for determination of tetrahydrotetrols of polycyclic aromatic hydrocarbons in acid hydrolysates of human hemoglobin has been investigated. A novel column-switching technique applying 'thermotransfer' is used to separate tetrahydrotetrols of benzo[a]pyrene and benz[a]anthracene more efficiently. Derivatives of polycyclic aromatic hydrocarbons from blood hydrolysates are concentrated on a pre-column and then transferred to the analytical column by applying an electrical current to heat the solvent eluting the pre-column. This method allows for rapid and quantitative transfer of the analytes from the pre-column to the analytical column, after HPLC-integrated sample processing.

引用

页码：153 / 159

页数：7

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