Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes

被引：32

作者：

Arrabito, Giuseppe ^{[2
]}

Galati, Clelia ^{[3
]}

Castellano, Sabrina ^{[4
]}

Pignataro, Bruno ^{[1
]}

机构：

[1] Univ Palermo, Dipartimento Chim S Cannizzaro, I-90128 Palermo, Italy

[2] Scuola Super Catania, I-95123 Catania, Italy

[3] ST Microelect, I-95123 Catania, Italy

[4] Univ Salerno, Dipartimento Sci Farmaceut & Biomed, I-84084 Fisciano, Italy

来源：

LAB ON A CHIP | 2013年 / 13卷 / 01期

关键词：

CYTOCHROME-P450; MICROARRAYS; FLEXIBILITY; PLATFORM; ASSAYS;

D O I：

10.1039/c2lc40948h

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for amultiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. the most relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.

引用

页码：68 / 72

页数：5

共 28 条

[1] Protein precipitation and denaturation by dimethyl sulfoxide [J].

Arakawa, Tsutomu ;

Kita, Yoshiko ;

Timasheff, Serge N. .

BIOPHYSICAL CHEMISTRY, 2007, 131 (1-3) :62-70

[2] Solution Processed Micro- and Nano-Bioarrays for Multiplexed Biosensing [J].

Arrabito, Giuseppe ;

Pignataro, Bruno .

ANALYTICAL CHEMISTRY, 2012, 84 (13) :5450-5462

[3] Inkjet Printing Methodologies for Drug Screening [J].

Arrabito, Giuseppe ;

Pignataro, Bruno .

ANALYTICAL CHEMISTRY, 2010, 82 (08) :3104-3107

[4] On the Relationship between Jetted Inks and Printed Biopatterns: Molecular-Thin Functional Microarrays of Glucose Oxidase [J].

Arrabito, Giuseppe ;

Musumeci, Chiara ;

Aiello, Venera ;

Libertino, Sebania ;

Compagnini, Giuseppe ;

Pignataro, Bruno .

LANGMUIR, 2009, 25 (11) :6312-6318

[5]

Aw KLD, 2010, METHODS MOL BIOL, V669, P79, DOI 10.1007/978-1-60761-845-4_7

[6] A Microwell Array Platform for Picoliter Membrane Protein Assays [J].

Binkert, Andreas ;

Studer, Philipp ;

Voeroes, Janos .

SMALL, 2009, 5 (09) :1070-1077

[7]

Cali JJ, 2008, EXPERT OPIN DRUG MET, V4, P103, DOI [10.1517/17425255.4.1.103, 10.1517/17425255.4.1.103 ]

[8] CYP19 (aromatase): Exploring the scaffold flexibility for novel selective inhibitors [J].

Castellano, Sabrina ;

Stefancich, Giorgio ;

Ragno, Rino ;

Schewe, Katarzyna ;

Santoriello, Marisabella ;

Caroli, Antonia ;

Hartmann, Rolf W. ;

Sbardella, Gianluca .

BIOORGANIC & MEDICINAL CHEMISTRY, 2008, 16 (18) :8349-8358

[9] Droplets for Ultrasmall-Volume Analysis [J].

Chiu, Daniel T. ;

Lorenz, Robert M. ;

Jeffries, Gavin D. M. .

ANALYTICAL CHEMISTRY, 2009, 81 (13) :5111-5118

[10] Electrochemical analysis in picoliter microvials [J].

Clark, RA ;

Hietpas, PB ;

Ewing, AG .

ANALYTICAL CHEMISTRY, 1997, 69 (02) :259-263

← 1 2 3 →