Precursor limitations in methyl jasmonate-induced Catharanthus roseus cell cultures

被引：29

作者：

Lee-Parsons, Carolyn W. T. ^{[1
]}

Royce, Amber J. ^{[1
]}

机构：

[1] Northeastern Univ, Dept Chem Engn, Snell Engn Ctr 342, Boston, MA 02115 USA

来源：

PLANT CELL REPORTS | 2006年 / 25卷 / 06期

基金：

美国国家科学基金会;

关键词：

ajmalicine; Catharanthus roseus; loganin; methyl jasmonate; precursor feeding; tryptamine;

D O I：

10.1007/s00299-005-0109-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced with MJ (100 mu M) on day 6 and fed loganin (0.30 mM), tryptamine (0.15 mM), loganin plus tryptamine, or geraniol (0.1-1.0 mM) on day 7. While MJ increased ajmalicine production by 3-fold, induced cultures were still limited by terpenoid precursors. However, both induced and non-induced cultures became tryptamine-limited with excess loganin. Geraniol feeding also increased ajmalicine production in non-induced cultures. But MJ appeared to increase geraniol availability in induced cultures, due presumably to the increased expression of Dxs with MJ addition.

引用

页码：607 / 612

页数：6

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