Chromophore-assisted laser inactivation in cell biology

被引：117

作者：

Jacobson, Ken ^{[1
,2
]}

Rajfur, Zenon ^{[1
]}

Vitriol, Eric ^{[1
]}

Hahn, Klaus ^{[2
,3
]}

机构：

[1] Univ N Carolina, Dept Cell & Dev Biol, Chapel Hill, NC 27599 USA

[2] Univ N Carolina, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA

[3] Univ N Carolina, Dept Pharmacol, Chapel Hill, NC 27599 USA

来源：

TRENDS IN CELL BIOLOGY | 2008年 / 18卷 / 09期

关键词：

D O I：

10.1016/j.tcb.2008.07.001

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. CALI is an important complement to conventional genetic and pharmacological manipulations. In this review, we examine the applications of CALI to cell biology and discuss the underlying photochemical mechanisms that mediate this powerful technique.

引用

页码：443 / 450

页数：8

共 45 条

[1] Reactive oxygen species: Metabolism, oxidative stress, and signal transduction [J].

Apel, K ;

Hirt, H .

ANNUAL REVIEW OF PLANT BIOLOGY, 2004, 55 :373-399

[2] QUENCHING OF SINGLET OXYGEN BY BIOMOLECULES FROM L1210 LEUKEMIA-CELLS [J].

BAKER, A ;

KANOFSKY, JR .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1992, 55 (04) :523-528

[3] Negative regulation of fibroblast motility by Ena/VASP proteins [J].

Bear, JE ;

Loureiro, JJ ;

Libova, I ;

Fässler, R ;

Wehland, J ;

Gertler, FB .

CELL, 2000, 101 (07) :717-728

[4] Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motility [J].

Bear, JE ;

Svitkina, TM ;

Krause, M ;

Schafer, DA ;

Loureiro, JJ ;

Strasser, GA ;

Maly, IV ;

Chaga, OY ;

Cooper, JA ;

Borisy, GG ;

Gertler, FB .

CELL, 2002, 109 (04) :509-521

[5]

Beck S, 2002, PROTEOMICS, V2, P247, DOI 10.1002/1615-9861(200203)2:3<247::AID-PROT247>3.0.CO

[6]

2-K

[7] A genetically encoded photosensitizer [J].

Bulina, ME ;

Chudakov, DM ;

Britanova, OV ;

Yanushevich, YG ;

Staroverov, DB ;

Chepurnykh, TV ;

Merzlyak, EM ;

Shkrob, MA ;

Lukyanov, S ;

Lukyanov, KA .

NATURE BIOTECHNOLOGY, 2006, 24 (01) :95-99

[8] Singlet oxygen-mediated damage to proteins and its consequences [J].

Davies, MJ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 305 (03) :761-770

[9] Major signal increase in fluorescence microscopy through dark-state relaxation [J].

Donnert, Gerald ;

Eggeling, Christian ;

Hell, Stefan W. .

NATURE METHODS, 2007, 4 (01) :81-86

[10]

DOUGHERTY TJ, 1978, CANCER RES, V38, P2628

← 1 2 3 4 5 →