Interactions of the Escherichia coli hydrogenase biosynthetic proteins:: HybG complex formation

被引：27

作者：

Butland, G

Zhang, JW

Yang, WH

Sheung, A

Wong, P

Greenblatt, JF

Emili, A

Zamble, DB

机构：

[1] Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada

[2] Univ Toronto, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada

来源：

FEBS LETTERS | 2006年 / 580卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

hydrogenase; metallocenter assembly; accessory protein interactions; nickel;

D O I：

10.1016/j.febslet.2005.12.063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Assembly of the active site of the [NiFe]-hydrogenase enzymes involves a multi-step pathway and the coordinated activity of many accessory proteins. To analyze complex formation between these factors in Escherichia coli, they were genomically tagged and native multi-protein complexes were isolated. This method validated multiple interactions reported in separate studies from several organisms and defined a new complex containing the putative chaperone HybG and the large subunit of hydrogenase 1 or 2. The complex also includes HypE and HypD, which interact with each other before joining the larger complex. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：677 / 681

页数：5

相关论文

共 30 条

[1]

Interplay between the specific chaperone-like proteins HybG and HypC in maturation of hydrogenases 1, 2, and 3 from Escherichia coli [J].

Blokesch, M ;

Magalon, A ;

Böck, A .

JOURNAL OF BACTERIOLOGY, 2001, 183 (09) :2817-2822

[2]

Analysis of the transcarbamoylation-dehydration reaction catalyzed by the hydrogenase maturation proteins HypF and HypE [J].

Blokesch, M ;

Paschos, A ;

Bauer, A ;

Reissmann, S ;

Drapal, N ;

Böck, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2004, 271 (16) :3428-3436

[3]

Metal insertion into NiFe-hydrogenases [J].

Blokesch, M ;

Paschos, A ;

Theodoratou, E ;

Bauer, A ;

Hube, M ;

Huth, S ;

Böck, A .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2002, 30 :674-680

[4]

Maturation of [NiFe]-hydrogenases in Escherichia coli:: The HypC cycle [J].

Blokesch, M ;

Böck, A .

JOURNAL OF MOLECULAR BIOLOGY, 2002, 324 (02) :287-296

[5]

The complex between hydrogenase-maturation proteins HypC and HypD is an intermediate in the supply of cyanide to the active site iron of [NiFe]-hydrogenases [J].

Blokesch, M ;

Albracht, SPJ ;

Matzanke, BF ;

Drapal, NM ;

Jacobi, A ;

Böck, A .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 344 (01) :155-167

[6]

Osprey: a network visualization system [J].

Breitkreutz, BJ ;

Stark, C ;

Tyers, M .

GENOME BIOLOGY, 2003, 4 (03)

[7]

Interaction network containing conserved and essential protein complexes in Escherichia coli [J].

Butland, G ;

Peregrín-Alvarez, JM ;

Li, J ;

Yang, WH ;

Yang, XC ;

Canadien, V ;

Starostine, A ;

Richards, D ;

Beattie, B ;

Krogan, N ;

Davey, M ;

Parkinson, J ;

Greenblatt, J ;

Emili, A .

NATURE, 2005, 433 (7025) :531-537

[8]

Interaction of the hydrogenase accessory protein HypC with HycE, the large subunit of Escherichia coli hydrogenase 3 during enzyme maturation [J].

Drapal, N ;

Böck, A .

BIOCHEMISTRY, 1998, 37 (09) :2941-2948

[9]

Assembly of Tat-dependent [NiFe] hydrogenases: identification of precursor-binding accessory proteins [J].

Dubini, A ;

Sargent, F .

FEBS LETTERS, 2003, 549 (1-3) :141-146

[10]

Network of hydrogenase maturation in Escherichia coli:: Role of accessory proteins HypA and HybF [J].

Hube, M ;

Blokesch, M ;

Böck, A .

JOURNAL OF BACTERIOLOGY, 2002, 184 (14) :3879-3885