Recognition of galactan components of pectin by galectin-3

被引：130

作者：

Gunning, A. Patrick ^{[1
]}

Bongaerts, Roy J. M. ^{[1
]}

Morris, Victor J. ^{[1
]}

机构：

[1] Inst Food Res, Norwich NR4 7UA, Norfolk, England

来源：

FASEB JOURNAL | 2009年 / 23卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

flow cytometry; AFM; antimetastasis activity; polygalacturonic acid; RGI; ATOMIC-FORCE MICROSCOPY; HUMAN BREAST-CARCINOMA; RHAMNOGALACTURONAN-I; CITRUS PECTIN; CELL-ADHESION; APOPTOSIS; ARABINOGALACTAN; INHIBITORS; CANCER; POLYSACCHARIDES;

D O I：

10.1096/fj.08-106617

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been reported that modified forms of pectin possess anticancer activity. To account for this bioactivity, it has been proposed that fragments of pectin molecules can act by binding to and inhibiting the various roles of the mammalian protein galectin 3 (Gal3) in cancer progression and metastasis. Despite this clear molecular hypothesis and evidence for the bioactivity of modified pectin, the structural origins of the "bioactive fragments" of pectin molecules are currently ill defined. By using a combination of fluorescence microscopy, flow cytometry, and force spectroscopy, it has been possible to demonstrate, for the first time, specific binding of a pectin galactan to the recombinant form of human Gal3. Present studies suggest that bioactivity resides in the neutral sugar side chains of pectin polysaccharides and that these components could be isolated and modified to optimize bioactivity. - Gunning, A. P., Bongaerts, R. J. M., Morris, V. J. Recognition of galactan components of pectin by galectin-3. FASEB J. 23, 415-424 (2009)

引用

页码：415 / 424

页数：10

共 40 条

[1]

[Anonymous], 1996, ALTERN MED REV

[2] GALECTINS - A FAMILY OF ANIMAL BETA-GALACTOSIDE-BINDING LECTINS [J].