The effect of immobilized platelet derived growth factor AA on neural stem/progenitor cell differentiation on cell-adhesive hydrogels

被引：64

作者：

Aizawa, Yukie ^{[1
]}

Leipzig, Nic ^{[1
]}

Zahir, Tasneem ^{[1
]}

Shoichet, Molly ^{[1
,2
,3
]}

机构：

[1] Univ Toronto, Dept Chem Engn & Appl Chem, Toronto, ON M5S 3E1, Canada

[2] Univ Toronto, Inst Biomat & Biomed Engn, Toronto, ON M5S 3E1, Canada

[3] Univ Toronto, Dept Chem, Toronto, ON M5S 3E1, Canada

来源：

BIOMATERIALS | 2008年 / 29卷 / 35期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Neural stem/progenitor cell differentiation; Hydrogel; Growth factor immobilization;

D O I：

10.1016/j.biomaterials.2008.08.018

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Neural stem/progenitor cells (NSPCs) hold great promise in regenerative medicine; however, controlling their differentiation to a desired phenotype within a defined matrix is challenging. To guide the differentiation of NSPCs, we first created a cell-adhesive matrix of agarose modified with glycine-arginine-glycine-aspartic acid-serine (GRGDS) and then demonstrated the multi potentiality of NSPCs to differentiate to the three primary cell types of the central nervous system on this matrix: neurons, oligodendrocytes and astrocytes. We then examined whether immobilized platelet derived growth factor AA (PDGF-AA) would promote differentiation similarly to the same soluble factor and found similar percentages of NSPCs differentiated to oligodendrocytes as determined by immunohistochemistry (IHC) and quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Interestingly, the gene expression of the differentiated oligodendrocytes was similar for 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) but different for myelin oligodendrocyte glycoprotein (MOG) in the presence of soluble PDGF-AA vs. immobilized PDGF-AA. These results demonstrate for the first time, that it is possible to control the differentiation of NSPCs, and specifically to oligodendrocytes, in cell-adhesive matrices with immobilized PDGF-AA. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：4676 / 4683

页数：8

共 35 条

[1] Neural stem cells: From cell biology to cell replacement [J].

Armstrong, RJE ;

Svendsen, CN .

CELL TRANSPLANTATION, 2000, 9 (02) :139-152

[2] Biology of oligodendrocyte and myelin in the mammalian central nervous system [J].

Baumann, N ;

Pham-Dinh, D .

PHYSIOLOGICAL REVIEWS, 2001, 81 (02) :871-927

[3] Protein patterning [J].

Blawas, AS ;

Reichert, WM .

BIOMATERIALS, 1998, 19 (7-9) :595-609

[4] Defining the concentration gradient of nerve growth factor for guided neurite outgrowth [J].

Cao, X ;

Shoichet, MS .

NEUROSCIENCE, 2001, 103 (03) :831-840

[5]

CHAMOW SM, 1992, J BIOL CHEM, V267, P15916

[6] Covalently immobilized gradients of bFGF on hydrogel scaffolds for directed cell migration [J].

DeLong, SA ;

Moon, JJ ;

West, JL .

BIOMATERIALS, 2005, 26 (16) :3227-3234

[7] Role of growth factors on periodontal repair [J].

Dereka, X. E. ;

Markopoulou, C. E. ;

Vrotsos, I. A. .

GROWTH FACTORS, 2006, 24 (04) :260-267

[8] Mammalian neural stem cells [J].

Gage, FH .

SCIENCE, 2000, 287 (5457) :1433-1438

[9] Long-term cultivation of multipotential neural stem cells from adult rat subependyma [J].

Gobbel, GT ;

Choi, SJ ;

Beier, S ;

Niranjan, A .

BRAIN RESEARCH, 2003, 980 (02) :221-232

[10] Platelet-derived growth factor-AA mediates oligodendrocyte lineage differentiation through activation of extracellular signal-regulated kinase signaling pathway [J].

Hu, J. -G. ;

Fu, S. -L. ;

Wang, Y. -X. ;

Li, Y. ;

Jiang, X. -Y. ;

Wang, X. -F. ;

Qiu, M. -S. ;

Lu, P. -H. ;

Xu, X. -M. .

NEUROSCIENCE, 2008, 151 (01) :138-147

← 1 2 3 4 →