Post-transcriptional down-regulation of Toll-like receptor signaling pathway in umbilical cord blood plasmacytoid dendritic cells

被引：21

作者：

Charrier, Emily ^{[1
,2
]}

Cordeiro, Paulo ^{[1
]}

Cordeau, Martine ^{[1
,3
]}

Dardari, R'Kia ^{[1
]}

Michaud, Annie ^{[1
,3
]}

Harnois, Michael ^{[1
,3
]}

Merindol, Natacha ^{[1
,3
]}

Herblot, Sabine ^{[1
,4
]}

Duval, Michel ^{[1
,2
,3
,4
]}

机构：

[1] CHU St Justine, Lab Sang Cordon, Ctr Cancerol Charles Bruneau, GRETISC,Ctr Rech, Montreal, PQ H3T 1C5, Canada

[2] Univ Montreal, Dept Biomed Sci, Montreal, PQ H3C 3J7, Canada

[3] Univ Montreal, Dept Microbiol & Immunol, Montreal, PQ H3C 3J7, Canada

[4] Univ Montreal, Dept Pediat, Montreal, PQ H3C 3J7, Canada

来源：

CELLULAR IMMUNOLOGY | 2012年 / 276卷 / 1-2期

关键词：

Human plasmacytoid dendritic cells; Cord blood; Innate immunity; Interferon-alpha; TLR pathway; VERSUS-HOST-DISEASE; BONE-MARROW; I IFN; MICRORNAS; RESPONSES; IMMUNITY; TRANSPLANTATION; INDUCTION; CHILDREN; DIFFERENTIATION;

D O I：

10.1016/j.cellimm.2012.04.010

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Plasmacytoid dendritic cells (PDCs) from human umbilical cord blood (UCB) produce lower amounts of IFN-alpha upon TLR stimulation compared with adult counterparts. This difference may play a role in the low graft-versus-host disease rate after UCB transplantation and in the impaired immune response of the neonate to pathogens. Comparing UCB PDC to their adults counterparts, we found that they exhibited a mature surface phenotype and a normal antigen uptake. They upregulated costimulatory molecules upon activation, although with delayed kinetics. Protein, but not ARN, levels of TLR-9, MyD88, IRAK1 and IRF-7, involved in the TLR-9 signaling pathway were reduced. The expression levels of miR-146a and miR-155, known to be involved in the post-transcriptional down-regulation of immune responses, were higher. These data point out a post-transcriptional down-regulation of the TLR-9/IRF-7 signaling pathway in UCB PDC. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：114 / 121

页数：8

共 54 条

[1] Role of plasmacytoid dendritic cells in immunity and tolerance after allogeneic hematopoietic stem cell transplantation [J].