Nitric oxide inhibits migration of cultured endothelial cells

被引:66
作者
Lau, YT
Ma, WC
机构
[1] Departments of Physiology, Chang Gung Coll. Med. and Technol., Kweisan
关键词
D O I
10.1006/bbrc.1996.0654
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Endothelial cell migration is an important event in both physiological and pathophysiological processes. Although nitric oxide (NO) plays a critical role in regulating vascular functions, it is not known whether NO modulates migration of endothelial cells. We show here that chemically-derived NO inhibited the serum-induced migration of cultured human umbilical vein endothelial cells (HUVEC) in a time- and dose-dependent manner. The sensitivity of inhibition by S-nitroso-N-acetylpenicillamine (SNAP, a NO donor) was 2.36+/-1.032x10(-4) M (n=4). This effect was attributed to NO since (1) other NO donor (e.g., sodium nitroprusside) also exhibited anitmigratory effect, (2) pre-incubated SNAP (72 h) had no effect, (3) hemoglobin, a NO scavenger, eliminated the effect; while (4) superoxide dismutase, a NO protector, enhanced the antimigratory effect. Furthermore, 8-bromo-cGMP also inhibited the serum-induced migration of HUVEC. These data appear to support the notion that NO may serve as an important signaling molecule for neovascularization. (C) 1996 Academic Press, Inc.
引用
收藏
页码:670 / 674
页数:5
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