Simultaneous determination of everolimus and cyclosporine concentrations by HPLC with ultraviolet detection

被引：36

作者：

Baldelli, S ^{[1
]}

Zenoni, S ^{[1
]}

Merlini, S ^{[1
]}

Perico, N ^{[1
]}

Cattaneo, D ^{[1
]}

机构：

[1] Osped Riuniti Bergamo, Mario Negri Inst Pharmacol Res, Dept Med & Transplantat, I-24125 Bergamo, Italy

来源：

CLINICA CHIMICA ACTA | 2006年 / 364卷 / 1-2期

关键词：

HPLC; cyclosporine; everolimus; therapeutic drug monitoring;

D O I：

10.1016/j.cca.2005.07.019

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

In the clinical practice of organ transplantation everolimus (RAD) is used in combination with cyclosporine (CsA), the most common antirejection agent. Both drugs show a narrow therapeutic window, which requires strict monitoring of their blood concentration. Simple methods for simultaneous measurement of RAD and CsA concentration are needed. As we have recently developed an HPLC-UV assay for RAD determination, we decided to implement it to allow concomitant measurement of CsA. The within- and between-day coefficients of variation of the measurement were less than 12.1% for RAD and 9.8% for CsA. The within- and between-day inaccuracy of quality control samples were less than 9.7% for RAD and less than 4.9% for CsA. The method was found accurate and precise and useful for simultaneous therapeutic monitoring of the two drugs. (C) 2005 Elsevier B.V All rights reserved.

引用

收藏

页码：354 / 358

页数：5

相关论文

共 20 条

[1] High-performance liquid chromatography with ultraviolet detection for therapeutic drug monitoring of everolimus [J].

Baldelli, S ;

Murgia, S ;

Merlini, S ;

Zenoni, S ;

Perico, N ;

Remuzzi, G ;

Cattaneo, D .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 816 (1-2) :99-105

[2] STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT [J].

BLAND, JM ;

ALTMAN, DG .

LANCET, 1986, 1 (8476) :307-310

[3] High-throughput semi-automated 96-well liquid/liquid extraction and liquid chromatography/mass spectrometric analysis of everolimus (RAD 001) and cyclosporin a (CsA) in whole blood [J].

Brignol, N ;

McMahon, LM ;

Luo, S ;

Tse, FLS .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2001, 15 (12) :898-907

[4] Comparison of different cyclosporine immunoassays to monitor C0 and C2 blood levels from kidney transplant recipients:: Not simply overestimation [J].

Cattaneo, D ;

Zenoni, S ;

Murgia, B ;

Merlini, S ;

Baldelli, S ;

Perico, N ;

Gotti, E ;

Ottomano, C ;

Crippa, A ;

Remuzzi, G .

CLINICA CHIMICA ACTA, 2005, 355 (1-2) :153-164

[5]

DEBRUYN CSV, 1986, CUMULATIVE SUM TESTS, P1

[6] Immunosuppressive drugs for kidney transplantation [J].

Halloran, PF .

NEW ENGLAND JOURNAL OF MEDICINE, 2004, 351 (26) :2715-2729

[7] Therapeutic drug monitoring of immunosuppressive drugs in kidney transplantation [J].

Holt, DW .

CURRENT OPINION IN NEPHROLOGY AND HYPERTENSION, 2002, 11 (06) :657-663

[8] LC/ESI-MS allows simultaneous and specific quantification of SDZ RAD and cyclosporine, including groups of their metabolites in human blood [J].

Kirchner, GI ;

Vidal, C ;

Winkler, M ;

Mueller, L ;

Jacobsen, W ;

Franzke, A ;

Sewing, KF .

THERAPEUTIC DRUG MONITORING, 1999, 21 (01) :116-122

[9] Simultaneous determination of four immunosuppressants by means of high speed and robust on-line solid phase extraction-high performance liquid chromatography-tandem mass spectrometry [J].

Koal, T ;

Deters, M ;

Casetta, B ;

Kaever, V .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 805 (02) :215-222

[10] Influence of delayed initiation of cyclosporine on everolimus pharmacokinetics in de novo renal transplant patients [J].

Kovarik, JM ;

Dantal, J ;

Civati, G ;

Rizzo, G ;

Rouilly, M ;

Bettoni-Ristic, O ;

Rordorf, C .

AMERICAN JOURNAL OF TRANSPLANTATION, 2003, 3 (12) :1576-1580