Aspergillus fumigatus C-5 sterol desaturases Erg3A and Erg3B:: Role in sterol biosynthesis and antifungal drug susceptibility

被引：38

作者：

Alcazar-Fuoli, L

Mellado, E

Garcia-Effron, G

Buitrago, MJ

Lopez, JF

Grimalt, JO

Cuenca-Estrella, JM

Rodriguez-Tudela, JL

机构：

[1] Inst Salud Carlos III, Ctr Nacl Microbiol, Serv Micol, Madrid, Spain

[2] CSIC, ICER, Dept Environm Chem, ES-08034 Barcelona, Spain

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2006年 / 50卷 / 02期

关键词：

D O I：

10.1128/AAC.50.2.453-460.2006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two erg3 genes encoding C-5 sterol desaturase enzymes (Erg3A and Erg3B) in Aspergillus fumigatus were characterized with respect to their nucleotide sequences and null mutant phenotypes. Targeted disruption of the erg3A and erg3B genes and a double gene knockout, erg3A(-) erg3B(-), showed that they are not essential for A. fumigatus viability. Mutant phenotypes clearly showed that Erg3B is a C-5 sterol desaturase, but no apparent role for Erg3A in A. fumigatus ergosterol biosynthesis was found. Susceptibility to amphotericin B, itraconazole, fluconazole, voriconazole, and ketoconazole was not altered in isolates in which erg3A and erg3B were knocked out alone and in combination.

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收藏

页码：453 / 460

页数：8

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