Enzymatic synthesis of mannosyl-cyclodextrin by alpha-mannosidase from jack bean

Mannosylated derivatives of cyclodextrins (CDs), mannosyl-alpha, beta, and gamma CD were synthesized from a mixture of mannose and alpha, beta, and gamma CD by the reverse action of alpha-mannosidase from jack bean, respectively, Their structures were analyzed by FAB-MS and C-13-NMR spectroscopies, and they were identified as 6-O-alpha-D-mannosyl-alpha, beta, land gamma CD. The optimum conditions for the production of 6-O-alpha-D-mannosyl-alpha CD by alpha-mannosidase were examined, Optimum pH and temperature were pH 4.5 and 60 degrees C, respectively, Yield of mannosyl-alpha CD increased with increasing mannose concentration and reached more than 35% (mol/mol) at the concentration of 2 M mannose and 0.4 M alpha CD.