T-cell responses to the Mycobacterium tuberculosis-specific antigens in active tuberculosis patients at the beginning, during, and after antituberculosis treatment

被引:45
作者
Dominguez, Jose [1 ,3 ,4 ]
De Souza-Galvao, Malu [3 ,5 ]
Ruiz-Manzano, Juan [2 ,3 ,4 ]
Latorre, Irene [1 ,3 ,4 ]
Prat, Cristina [1 ,3 ,4 ]
Lacoma, Alicia [1 ,3 ]
Mila, Celia [5 ]
Angeles Jimenez, Maria [5 ]
Blanco, Silvia [1 ,3 ,4 ]
Maldonado, Jose [6 ]
Altet, Neus [5 ]
Ausina, Vicente [1 ,3 ,4 ]
机构
[1] Hosp Badalona Germans Trias & Pujol, Microbiol Serv, Fdn Inst Invest Ciencies Salut Germans Trias & Pu, Barcelona 08916, Spain
[2] Hosp Badalona Germans Trias & Pujol, Serv Pneumol, Fdn Inst Invest Ciencies Salut Germans Trias & Pu, Barcelona 08916, Spain
[3] Univ Autonoma Barcelona, E-08193 Barcelona, Spain
[4] Inst Salud Carlos III, Badalona, Spain
[5] Unidad Prevenc & Control TB Barcelona, Barcelona, Spain
[6] Serv Clin Barcelona, Barcelona, Spain
关键词
Active tuberculosis; Mycobacterium tuberculosis; INTERFERON-GAMMA ASSAY; LINKED IMMUNOSPOT ASSAY; RELEASE ASSAYS; IFN-GAMMA; BLOOD-TESTS; SKIN-TEST; DIAGNOSIS; INFECTION; DISEASE; ESAT-6;
D O I
10.1016/j.diagmicrobio.2008.09.010
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The objectives of the study were to assess the performance of the QuantiFERON-TB Gold In-Tube (QFN-G-IT) and the T-SPOT.TB tests in the immunodiagnosis of active tuberculosis (TB) in adult patients, and to Study the T-cell interferon gamma (IFN-gamma) responses during treatment and in patients who have recovered after curative treatment and self-healed TB patients. When only analyzing patients included at the beginning of treatment, the sensitivity was 83.3% for T-SPOT.TB and 69.4% for QFN-G-IT. In contrast, when evaluating patients during treatment, the sensitivity of the T-SPOT.TB and QFN-G-IT decreased to 69.8% and 48.8%, respectively. The response to the specific antigens increased after finishing the treatment compared with the values during the treatment. The T-SPOT.TB was more sensitive in diagnosing active TB than the QFN-G-IT. The IFN-gamma tests could be used as a complementary method in the diagnosis of active TB. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:43 / 51
页数:9
相关论文
共 43 条
[1]   Specific immune-based diagnosis of tuberculosis [J].
Andersen, P ;
Munk, ME ;
Pollock, JM ;
Doherty, TM .
LANCET, 2000, 356 (9235) :1099-1104
[2]   CYTOKINE PRODUCTION AT THE SITE OF DISEASE IN HUMAN TUBERCULOSIS [J].
BARNES, PF ;
LU, SZ ;
ABRAMS, JS ;
WANG, E ;
YAMAMURA, M ;
MODLIN, RL .
INFECTION AND IMMUNITY, 1993, 61 (08) :3482-3489
[3]   Specific T-cell epitopes for immunoassay-based diagnosis of Mycobacterium tuberculosis infection [J].
Brock, I ;
Weldingh, K ;
Leyten, EMS ;
Arend, SM ;
Ravn, P ;
Andersen, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (06) :2379-2387
[4]   Use of a T cell-based assay for monitoring efficacy of antituberculosis therapy [J].
Carrara, S ;
Vincenti, D ;
Petrosillo, N ;
Amicosante, M ;
Girardi, E ;
Goletti, D .
CLINICAL INFECTIOUS DISEASES, 2004, 38 (05) :754-756
[5]   Comparison of sensitivities of two commercial gamma interferon release assays for pulmonary tuberculosis [J].
Chee, Cynthia B. E. ;
Gan, Suay H. ;
KhinMar, Kyi W. ;
Barkham, Timothy M. ;
Koh, Chwee K. ;
Liang, Shen ;
Wang, Yee T. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (06) :1935-1940
[6]   CD4+CD25+FoxP3+ regulatory T cells suppress Mycobacterium tuberculosis immunity in patients with active disease [J].
Chen, Xinchun ;
Zhou, Boping ;
Li, Meizhong ;
Deng, Qunyi ;
Wu, Xueqiong ;
Le, Xiaohua ;
Wu, Chi ;
Larmonier, Nicolas ;
Zhang, Wei ;
Zhang, Hongmei ;
Wang, Huosheng ;
Katsanis, Emmanuel .
CLINICAL IMMUNOLOGY, 2007, 123 (01) :50-59
[7]   Low sensitivity of a whole-blood interferon-γ release assay for detection of active tuberculosis [J].
Dewan, Puneet K. ;
Grinsdale, Jennifer ;
Kawamura, L. Masae .
CLINICAL INFECTIOUS DISEASES, 2007, 44 (01) :69-73
[8]   Interpretation of Mycobacterium tuberculosis antigen-specific IFN-γ release assays (T-SPOT.TB) and factors that may modulate test results [J].
Dheda, K. ;
Pooran, A. ;
Pai, M. ;
Miller, R. F. ;
Lesley, K. ;
Booth, H. L. ;
Scott, G. M. ;
Akbar, A. N. ;
Zumla, A. ;
Rook, G. A. .
JOURNAL OF INFECTION, 2007, 55 (02) :169-173
[9]   Comparison of two commercially available gamma interferon blood tests for immunodiagnosis of tuberculosis [J].
Dominguez, Jose ;
Ruiz-Manzano, Juan ;
De Souza-Galvao, Malu ;
Latorre, Irene ;
Mila, Celia ;
Blanco, Silvia ;
Angeles Jimenez, M. ;
Prat, Cristina ;
Lacoma, Alicia ;
Altet, Neus ;
Ausina, Vicente .
CLINICAL AND VACCINE IMMUNOLOGY, 2008, 15 (01) :168-171
[10]   Improved diagnostic evaluation of suspected tuberculosis [J].
Dosanjh, Davinder P. S. ;
Hinks, Timothy S. C. ;
Innes, John A. ;
Deeks, Jonathan J. ;
Pasvol, Geoffrey ;
Hackforth, Sarah ;
Varia, Hansa ;
Millington, Kerry A. ;
Gunatheesan, Rubamalar ;
Guyot-Revol, Valerie ;
Lalvani, Ajit .
ANNALS OF INTERNAL MEDICINE, 2008, 148 (05) :325-W72