Development of a surface acoustic wave immunosensor

被引：61

作者：

Welsch, W ^{[1
]}

Klein, C ^{[1
]}

vonSchickfus, M ^{[1
]}

Hunklinger, S ^{[1
]}

机构：

[1] UNIV HEIDELBERG, INST ANGEW PHYS, D-69120 HEIDELBERG, GERMANY

来源：

ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 13期

关键词：

D O I：

10.1021/ac960198z

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper the first application of horizontal polarized surface acoustic waves (HP-SAW) in LiTaO3 for an immunosensor is presented, A SAW device with two paths, a sensitive path and a reference path operating in liquid at 345 MHz, is used as the sensor, Antigens and antibodies were both immunoglobulins. The antibodies were immobilized via protein A on the sensitive path with a mass density of 0.4 ng/mm(2), as shown by ELISA measurements, A theoretical detection limit of similar to 33 pg and a high sensitivity of 110 kHz/(ng/mm(2)) could be derived from the experiments, High signal enhancement factors of 4.5 and 12 are achievable by gold colloid labeled antigens with colloid diameters of 5 and 10 nm, respectively. Specificity of the antibody was demonstrated by a preabsorption experiment. As a further antibody/antigen system the binding of the anti-human serum albumin (HSA) to HSA was examined.

引用

页码：2000 / 2004

页数：5

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