N-hexanoyl chitosan-stabilized magnetic nanoparticles: enhancement of adenoviral-mediated gene expression both in vitro and in vivo

被引：38

作者：

Bhattarai, Shanta Raj ^{[3
]}

Kim, Sun Young ^{[1
,2
]}

Jang, Kyu Yun ^{[4
]}

Lee, Ki Chang ^{[5
]}

Yi, Ho Keun ^{[6
]}

Lee, Dae Yeol ^{[1
,2
]}

Kim, Hak Yong ^{[7
]}

Hwang, Pyoung Han ^{[1
,2
]}

机构：

[1] Chonbuk Natl Univ, Dept Pediat, Sch Med, Jeonju 561712, Jeonbuk, South Korea

[2] Chonbuk Natl Univ, Res Inst Clin Med, Sch Med, Jeonju 561712, Jeonbuk, South Korea

[3] Wayne State Univ, Dept Pharmaceut Sci, Detroit, MI USA

[4] Chonbuk Natl Univ, Sch Med, Dept Pathol, Jeonju 561712, Jeonbuk, South Korea

[5] Chonbuk Natl Univ, Sch Vet Med, Dept Vet Radiol, Jeonju 561712, Jeonbuk, South Korea

[6] Chonbuk Natl Univ, Sch Dent, Dept Biochem, Jeonju 561712, Jeonbuk, South Korea

[7] Chonbuk Natl Univ, Dept Text Engn, Jeonju 561712, Jeonbuk, South Korea

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2008年 / 4卷 / 02期

关键词：

Magnetofection; Gene; Vectors; Magnetic nanoparticles; Chitosan; Iron oxide; Virus;

D O I：

10.1016/j.nano.2008.02.001

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

We developed hexanoyl chloride-modified chitosan (Nac-6) stabilized iron oxide nanoparticles (Nac-6-IOPs) as magnetic nanoparticles for viral gene (Ad/LacZ) delivery via magnetofection. This vector, Nac-6-IOPs/Ad/LacZ, binds to K562 cells in the presence of external magnetic fields and results in enhanced expression of the transgene in those cells that do not exhibit the coxsackie-adenovirus receptor (CAR). Our results demonstrate that Nac-6-IOPs/Ad/LacZ is able to transduce K562 cells specifically with reduced infection of CAR-cells. The dramatic enhancement in intracellular trafficking of the adenovirus without genetically modified vesicles can lead to enhanced nuclear transfer, especially in CAR-cells. In vivo magnetofection results also clearly demonstrated that the present Nac-6-IOPs could be applied in other cell lines. Whether cells or organs, in the presence of magnetic fields Nac-6-IOPs/Ad/LacZ has high transduction efficiency. The newly formulated Nac-6-IOPs, introduced by magnetofection, provide a high-throughput gene screening both in vitro and in vivo. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：146 / 154

页数：9

共 20 条

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