One-step preparation of antibody and oligonucleotide dual-labeled gold nanoparticle bio-probes and their properties

被引：13

作者：

Kong, Xiao-li ^{[1
]}

Qiao, Fei-yan ^{[1
]}

Qi, Hui ^{[1
]}

Li, Fu-rong ^{[1
]}

机构：

[1] Jinan Univ, Shenzhen Peoples Hosp, Clin Med Coll 2, Clin Med Res Ctr, Shenzhen 518020, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 12期

关键词：

Antibody; Gold nanoparticle; Nanoparticles; Oligonucleotide; Probe;

D O I：

10.1007/s10529-008-9802-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel method of one-step preparation of dual-labeled gold nanoparticle bio-probes was established by the electrostatic adsorption and the covalent bonding of gold nanoparticles with antibodies and thiol-modified oligonucleotides, respectively. Characterization of probes, the coverage and activity of antibodies and oligonucleotides on probe surfaces were detected. The results indicated that the gold nanoparticles labeled with antibodies and oligonucleotides possess good bioactivity and the coverage of oligonucleotide and antibody on a dual-labeled gold nanoparticle bio-probe was (92 +/- 20) and (8 +/- 3), respectively. The preparative method is simple and stable. The dual-labeled gold nanoparticle bio-probes have an application value in detection of ultramicro protein.

引用

页码：2071 / 2077

页数：7

共 14 条

[11] Nanoparticle-DNA conjugates bearing a specific number of short DNA strands by enzymatic manipulation of nanoparticle-bound DNA [J].

Qin, WJ ;

Yung, LYL .

LANGMUIR, 2005, 21 (24) :11330-11334

[12] Multiplexed detection of protein cancer markers with biobarcoded nanoparticle probes [J].

Stoeva, Savka I. ;

Lee, Jae-Seung ;

Smith, Jennifer E. ;

Rosen, Steven T. ;

Mirkin, Chad A. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (26) :8378-8379

[13] Multiplexed DNA detection with biobarcoded nanoparticle probes [J].

Stoeva, Savka I. ;

Lee, Jae-Seung ;

Thaxton, C. Shad ;

Mirkin, Chad A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (20) :3303-3306

[14] Plugging into enzymes: Nanowiring of redox enzymes by a gold nanoparticle [J].

Xiao, Y ;

Patolsky, F ;

Katz, E ;

Hainfeld, JF ;

Willner, I .

SCIENCE, 2003, 299 (5614) :1877-1881

← 1 2 →