Expression and properties of sorting nexin 9 in dynamin-mediated endocytosis

被引：9

作者：

Lundmark, R ^{[1
]}

Carlsson, SR ^{[1
]}

机构：

[1] Umea Univ, Dept Med Biochem & Biophys, Umea, Sweden

来源：

GTPASES REGULATING MEMBRANE DYNAMICS | 2005年 / 404卷

关键词：

D O I：

10.1016/S0076-6879(05)04048-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sorting nexin 9 (SNX9) is identified as an important regulator of dynamin function in clathrin-mediated endocytosis. SNX9 recruits dynamin to the plasma membrane and promotes its GTPase activity, resulting in membrane constriction and ultimate transport vesicle scission. This chapter describes procedures to express recombinant SNX9, to biochemically characterize the cytosolic complex between SNX9 and dynamin, and to identify additional interacting partners of SNX9. Assays are presented to investigate the requirements for SNX9-dependent membrane recruitment of dynamin in vitro and in vivo.

引用

页码：545 / 556

页数：12

共 7 条

[1] Sorting nexins - Unifying trends and new perspectives [J].