Molecular evolution of two vertebrate aryl hydrocarbon (dioxin) receptors (AHR1 and AHR2) and the PAS family

被引:226
作者
Hahn, ME
Karchner, SI
Shapiro, MA
Perera, SA
机构
[1] Biology Department, Woods Hole Oceanographic Institution, Woods Hole
[2] Biology Department, Redfield 338 (MS# 32), Woods Hole Oceanographic Institution, Woods Hole
关键词
D O I
10.1073/pnas.94.25.13743
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor through which halogenated aromatic hydrocarbons such as 2,3,7,8-tetrachloro-dibenzo-p-dioxin (TCDD) cause altered gene expression and toxicity. The AHR belongs to the basic helix-loop-helix/Per-ARNT-Sim (bHLH-PAS) family of transcriptional regulatory proteins, whose members play key roles in development, circadian rhythmicity, and environmental homeostasis; however, the normal cellular function of the AHR is not yet known. As part of a phylogenetic approach to understanding the function and evolutionary origin of the AHR, we sequenced the PAS homology domain of AHRs from several species of early vertebrates and performed phylogenetic analyses of these AHR amino acid sequences in relation to mammalian AHRs and 24 other members of the PAS family. AHR sequences were identified in a teleost (the killifish Fundulus heteroclitus), two elasmobranch species (the skate Raja erinacea and the dogfish Mustelus canis), and a jawless fish (the lamprey Petromyzon marinus). Two putative AHR genes, designated AHR1 and AHR2, were found both in Fundulus and Mustelus. Phylogenetic analyses indicate that the AHR2 genes in these two species are orthologous, suggesting that an AHR gene duplication occurred early in vertebrate evolution and that multiple AHR genes may be present in other vertebrates. Database searches and phylogenetic analyses identified four putative PAS proteins in the nematode Caenorhabditis elegans, including possible AHR and ARNT homologs. Phylogenetic analysis of the PAS gene family reveals distinct clades containing both invertebrate and vertebrate PAS family members; the latter include paralogous sequences that we propose have arisen by gene duplication early in vertebrate evolution. Overall, our analyses indicate that the AHR is a phylogenetically ancient protein present in all living vertebrate groups (with a possible invertebrate homolog), thus providing an evolutionary perspective to the study of dioxin toxicity and AHR function.
引用
收藏
页码:13743 / 13748
页数:6
相关论文
共 60 条
[1]   MOLECULAR EVOLUTION OF THE MYOD FAMILY OF TRANSCRIPTION FACTORS [J].
ATCHLEY, WR ;
FITCH, WM ;
BRONNERFRASER, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (24) :11522-11526
[2]   A natural classification of the basic helix-loop-helix class of transcription factors [J].
Atchley, WR ;
Fitch, WM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (10) :5172-5176
[3]   MYC AND MAX - MOLECULAR EVOLUTION OF A FAMILY OF PROTOONCOGENE PRODUCTS AND THEIR DIMERIZATION PARTNER [J].
ATCHLEY, WR ;
FITCH, WM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (22) :10217-10221
[4]   CLONING OF THE AH-RECEPTOR CDNA REVEALS A DISTINCTIVE LIGAND-ACTIVATED TRANSCRIPTION FACTOR [J].
BURBACH, KM ;
POLAND, A ;
BRADFIELD, CA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (17) :8185-8189
[5]   HOMEOTIC GENES AND THE EVOLUTION OF ARTHROPODS AND CHORDATES [J].
CARROLL, SB .
NATURE, 1995, 376 (6540) :479-485
[6]   DEFINITION OF A MINIMAL DOMAIN OF THE DIOXIN RECEPTOR THAT IS ASSOCIATED WITH HSP90 AND MAINTAINS WILD-TYPE LIGAND-BINDING AFFINITY AND SPECIFICITY [J].
COUMAILLEAU, P ;
POELLINGER, L ;
GUSTAFSSON, JA ;
WHITELAW, ML .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (42) :25291-25300
[7]   THE DROSOPHILA SINGLE-MINDED GENE ENCODES A NUCLEAR-PROTEIN WITH SEQUENCE SIMILARITY TO THE PER GENE-PRODUCT [J].
CREWS, ST ;
THOMAS, JB ;
GOODMAN, CS .
CELL, 1988, 52 (01) :143-151
[8]   Neurospora wc-1 and wc-2: Transcription, photoresponses, and the origins of circadian rhythmicity [J].
Crosthwaite, SK ;
Dunlap, JC ;
Loros, JJ .
SCIENCE, 1997, 276 (5313) :763-769
[9]   IN-VITRO ANALYSIS OF AH RECEPTOR DOMAINS INVOLVED IN LIGAND-ACTIVATED DNA RECOGNITION [J].
DOLWICK, KM ;
SWANSON, HI ;
BRADFIELD, CA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (18) :8566-8570
[10]   A novel bHLH-PAS factor with close sequence similarity to hypoxia-inducible factor 1 alpha regulates the VEGF expression and is potentially involved in lung and vascular development [J].
Ema, M ;
Taya, S ;
Yokotani, N ;
Sogawa, K ;
Matsuda, Y ;
FujiiKuriyama, Y .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (09) :4273-4278